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gp49B1-αvβ3 interaction inhibits antigen-induced mast cell activation

Nature Immunology volume 2, pages 436–442 (2001)Cite this article

Abstract

We have identified the integrin αvβ3 as a ligand for mouse gp49B1, thus identifying a new class of ligand for a member of the family of inhibitory immunoreceptors that bear C2-type immunoglobulin-like domains. The specific interaction was shown by both cell-protein and cell-cell binding assays. In addition, we found that the interaction of αvβ3 with gp49B1 on bone marrow–derived mouse mast cells inhibited antigen-induced immunoglobulin E–mediated cell activation. Because neither gp49B1 nor αvβ3 exhibit substantive allelic variation, their newly appreciated interaction may reflect an innate pathway for down-regulating the activity of mast cells.

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Figure 1: Aggregation of T cells with P815.gp49B1 transfectants.
Figure 2: Binding of BY155.16 cells and human K562. αvβ3 transfectants to plate-bound gp49B1-Fc.
Figure 3: Binding of mouse P815.gp49B1 transfectants to plate-bound human αvβ3 and inhibition of vitronectin binding to αvβ3 by gp49B1-Fc.
Figure 4: Cell-cell interactions between BMMCs expressing gp49B1 and BY155.16 cells expressing αvβ3.
Figure 5: Inhibition of IgE-dependent BMMC activation by solid-phase αvβ3.

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Acknowledgements

We thank M. Brenner and C. Parker for advice and X.-Y. Wu, L. Rodriguez, B. Sampson, A. Bass, E.Wasser and M. Diaz Jara for technical assistance. Supported by NIH grants AI-01414, AI-07306, AI-22531, AI-31599, AI-41144, AR-45025, AR-47243 and HL-36110 and by grants from the Hyde and Watson Foundation, Arthritis Foundation and Osteogenesis Imperfecta Foundation.

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  1. Department of Medicine, Harvard Medical School and Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Boston, 02115, MA, USA

    Mariana C. Castells, Lloyd B. Klickstein, Kevin Hassani, Jose A. Cumplido, Mario E. Lacouture, K. Frank Austen & Howard R. Katz

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  1. Mariana C. Castells
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Correspondence to Mariana C. Castells.

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Castells, M., Klickstein, L., Hassani, K. et al. gp49B1-αvβ3 interaction inhibits antigen-induced mast cell activation. Nat Immunol 2, 436–442 (2001). https://doi.org/10.1038/87749

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