From totipotency to T in a dish

Given the right sequence of cues, embryonic stem cells can develop into any cell type found in the body. New work shows how manipulation of supportive stromal cells to provide essential Delta-Notch interactions allows embryonic stem cells to develop into functional T lymphocytes during in vitro culture.

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Figure 1: Development of T and B lymphocytes from embryonic stem cells in vitro using OP9 and OP9-DL1 stromal lines.

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Rothenberg, E. From totipotency to T in a dish. Nat Immunol 5, 359–360 (2004). https://doi.org/10.1038/ni0404-359

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