Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Creating new peptide antigens by slicing and splicing proteins

The proteome universe may be best represented by the peptides caught by major histocompatibility complex proteins. Unusual peptides, such as the post-translationally spliced example seen by the authors of a recent Nature paper, would not be included in a protein world gleaned only from genomics.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Figure 1: Post-translational modifications of proteins that determine the structure of class I associated peptides.



  1. Hanada, K., Yewdell, J.W. & Yang, J.C. Nature 427, 252–256 (2004).

    Article  CAS  Google Scholar 

  2. Paulus, H. Annu. Rev. Biochem. 69, 447–496 (2000).

    Article  CAS  Google Scholar 

  3. Min, W. & Jones, D.H. Nat. Struct. Biol. 1, 502–504 (1994).

    Article  CAS  Google Scholar 

  4. Yewdell, J.W. Mol. Immunol. 39, 139–146 (2002).

    Article  CAS  Google Scholar 

  5. Shastri, N., Schwab, S. & Serwold, T. Annu. Rev. Immunol. 20, 463–493 (2002).

    Article  CAS  Google Scholar 

  6. Robbins, P.F. et al. J. Immunol. 154, 5944–5950 (1995).

    CAS  PubMed  Google Scholar 

  7. Schwab, S.R., Li, K.C., Kang, C. & Shastri, N. Science 301, 1367–1371 (2003).

    Article  CAS  Google Scholar 

  8. Engelhard, V.H., Brickner, A.G. & Zarling, A.L. Mol. Immunol. 39, 127–137 (2002).

    Article  CAS  Google Scholar 

  9. Martin, S.E., Shabanowitz, J., Hunt, D.F. & Marto, J.A. Anal. Chem. 72, 4266–4274 (2000).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and Permissions

About this article

Cite this article

Engelhard, V. Creating new peptide antigens by slicing and splicing proteins. Nat Immunol 5, 128–129 (2004).

Download citation

  • Issue Date:

  • DOI:


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing