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A gammaherpesvirus provides protection against allergic asthma by inducing the replacement of resident alveolar macrophages with regulatory monocytes

A Correction to this article was published on 28 June 2018

Abstract

The hygiene hypothesis postulates that the recent increase in allergic diseases such as asthma and hay fever observed in Western countries is linked to reduced exposure to childhood infections. Here we investigated how infection with a gammaherpesvirus affected the subsequent development of allergic asthma. We found that murid herpesvirus 4 (MuHV-4) inhibited the development of house dust mite (HDM)-induced experimental asthma by modulating lung innate immune cells. Specifically, infection with MuHV-4 caused the replacement of resident alveolar macrophages (AMs) by monocytes with regulatory functions. Monocyte-derived AMs blocked the ability of dendritic cells to trigger a HDM-specific response by the TH2 subset of helper T cells. Our results indicate that replacement of embryonic AMs by regulatory monocytes is a major mechanism underlying the long-term training of lung immunity after infection.

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Figure 1: Infection with MuHV-4 inhibits the development of HDM-induced allergic asthma.
Figure 2: Infection with MuHV-4 affects the priming of HDM-induced TH2 responses.
Figure 3: Infection with MuHV-4 affects the maturation of MLN DCs.
Figure 4: Infection with MuHV-4 affects the phenotype of AMs.
Figure 5: MuHV4 induces the replacement of resident AMs by regulatory monocytes.
Figure 6: Monocyte-derived AMs persist long term in MuHV-4-infected mice.
Figure 7: AMs from MuHV-4-infected mice are necessary and sufficient for blocking HDM-induced airway allergy.
Figure 8: 'MuHV-4-imprinted' AMs inhibit the induction of airway allergy by HDM-pulsed BMDCs.

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Acknowledgements

We thank U. Eriksson (Center for Molecular Cardiology, University of Zurich) for BALB/c CD45.1+ genitor mice; A. Osterhaus, T. Marichal and C. Desmet for critical discussions; and L. Dams, C. Delforge, E. Deglaire, C. Espert, A. Guillaume, M. Sarlet and A. Vanderlinden for technical and secretary assistance. Supported by the University of Liège (VIR-IMPRINT ARC), “Fonds de la Recherche Scientifique - Fonds National Belge de la Recherche Scientifique” (“credit de recherche” J007515F; “projet de recherche” T.0195.16; research associate support for B.D.) and Institut MERIEUX (starting grant).

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Authors

Contributions

B.M., M.D. and L.G. designed the experiments with the help of H.H., M.G., B.N.L. and F.B.; B.M. and M.D. did most of the experiments and compiled the data; B.M., M.D., X.X. and L.G. prepared the figures; X.X. performed the transcriptomic and statistical analyses; J.J., C.M., C.S., F.L., and P.M. were involved in specific experiments; B.M., M.D., D.D., H.H., M.G., B.D., A.V., B.N.L., F.B. and L.G. analyzed the data; and B.M., M.D. and L.G. wrote the manuscript.

Corresponding author

Correspondence to Laurent Gillet.

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The authors declare no competing financial interests.

Integrated supplementary information

Supplementary Figure 1 Infection with MuHV-4 protects both BALB/c and C57BL/6 mice against HDM-induced allergic asthma.

(a) Experimental design of HDM-induced high dose model of asthma in BALB/c mice. Mice were mock-infected or infected intranasally with MuHV-4 (1x104 PFU in 50 μL PBS). Thirty days post-infection (p.i.), animals received 3 sequential intranasal instillations of saline or 100 μg HDM extracts (day 0, 7, 14) before euthanasia and airway allergy evaluation 3 days later (day 17). (b) Experimental design of HDM-induced low dose model of asthma in BALB/c or C57BL/6. Thirty days p.i. as in a, animals received intranasal saline instillation or were sensitized with 10 μg HDM (day 0). One week later, mice were challenged with 5 daily intranasal instillations of 10 μg HDM (days 7 to 11) before euthanasia and airway allergy evaluation 3 days after the last instillation (day 14). (c-g) Total and differential cell counts of BALF cells (c,e), ELISA measurement of cytokine release by MLN cells following ex vivo restimulation with HDM (d,f), and ELISA Measurement of HDM-specific IgE, IgG1 and IgG2c levels in sera (g) at euthanasia of mock or MuHV-4-infected BALB/c (c-d) or C57BL/6 mice (e-g), submitted 30 days p.i. to the HDM-induced low dose model of asthma (as in b). *** p< 0.001, ** p< 0.01 and * p < 0.05 (Two-way ANOVA and Tukey's multiple comparison test (c-e) or Student's t-test (g)). Data are representative of 2 independent experiments with 5 mice per group (mean ± s.e.m. in c-g).

Supplementary Figure 2 Infection with MuHV-4 impairs HDM-induced airway allergy in young mice.

(a-d) Quantification of MuHV-4 genomic copies in splenic cells by qPCR (a) and of MuHV-4 specific immunoglobulins in sera by ELISA (b), histological analysis of lung sections (scale bars, 100 μm) (c), total and differential cell counts of BALF cells (d), at euthanasia of mock or MuHV-4-infected 3 weeks old BALB/c mice, submitted 30 days p.i. to the HDM-induced high dose model of asthma. Data are mean ± s.e.m. of samples from 5 mice per group. *** p< 0.001, ** p< 0.01 and * p < 0.05 (Two-way (a, b) or One-way (d) ANOVA and Tukey's multiple comparison test).

Supplementary Figure 3 Infection with MuHV-4 induces a long-term protection against HDM-induced airway allergy.

(a-d) Quantification of MuHV-4 specific immunoglobulins in sera by ELISA (a), total and differential cell counts of BALF cells (b), histological analysis of lung sections (scale bars, 100 μm) (c), and ELISA measurement of cytokine release by MLN cells following ex vivo restimulation by HDM (d), at euthanasia of mock or MuHV-4-infected BALB/c mice, submitted 7, 30 or 60 days p.i. to the HDM-induced high dose model of asthma. *** p< 0.001, ** p< 0.01 and * p < 0.05 (Two-way ANOVA and Tukey's multiple comparison test). Data are representative of two independent experiments with 5 mice per group (mean ± s.e.m. in a, b, d).

Supplementary Figure 4 Establishment of MuHV-4 latency is not necessary to allow protection against HDM-induced airway allergy.

(a-d) Quantification of MuHV-4 specific immunoglobulins in sera by ELISA (a), quantification of MuHV-4 genomic copies in splenic cells by qPCR (b), histological analysis of lung sections (scale bars, 100 μm) (c), and total and differential cell counts of BALF cells (d) at euthanasia of mock or MuHV-4-infected BALB/c mice either with the WT strain or with latency-deficient viral mutants (FS73 and Del73 strains) or with a corresponding revertant (Rev73), submitted 30 days p.i. to the HDM-induced high dose model of asthma. *** p< 0.001, ** p< 0.01 and * p < 0.05 (Two-way ANOVA and Tukey's multiple comparison test). Data are representative of 2 independent experiments with 5 mice per group (mean ± s.e.m. in a,b,d).

Supplementary Figure 5 Migratory DC subsets in MLNs from mock- and MuHV-4 infected mice after HDM sensitization.

(a) Gating strategy for CD103+ cDC1, CD11b+ cDC2, lung-derived CCR7+ DC populations in the MLN of mock or MuHV-4 infected BALB/c mice, HDM sensitized (100 μg) 30 days p.i. and euthanized 2 days later. MLN cells from MuHV-4 infected mice were labelled with CFSE and then mixed with MLN cells from mock-infected mice prior to antibody staining and flow cytometry analysis of a single mix, allowing unbiased comparisons. Debris and doublets were excluded based on FSC and SSC. MOs were excluded based on Ly6c and CD64 expression. Migratory lung-derived CD11b+ cDC2 were identified as liveCD11c+MHC-IIhiCCR7+CD11b+ cells. Migratory lung-derived CD103+ cDC1s were identified as liveCD11c+MHC-IIhiCCR7+CD103+CD11blo cells. Representative flow cytometry plots are shown with the mean frequency of the different cells subsets. (b) Mean fluorescence intensities of maturation markers (CD40, CD80 and CD86, with independent staining and analysis for each of these markers) and MHCII by migratory DCs subsets were compared between the CFSE+ (originating from MuHV-4 infected mice) and CFSE- (originating from mock-infected mice) populations. A reciprocal experiment comparing CFSE+ DCs from mock-infected mice to CFSE- DCs from MuHV-4 infected has been performed as control and gave similar results (not shown).

Supplementary Figure 6 Intranasal infection with MuHV-4 protects mice against HDM-induced airway allergy, but intraperitoneal infection does not.

(a-d) Quantification of MuHV-4 genomic copies in splenic cells by qPCR (a), quantification of MuHV-4 specific immunoglobulins in sera by ELISA (b), total and differential cell counts of BALF cells (d), and ELISA measurement of cytokine release by MLN cells following ex vivo restimulation by HDM (d) at euthanasia of mock, or MuHV-4 infected BALB/c mice either intranasally or intraperitoneally (1x104 PFU), submitted 30 days p.i. to the HDM-induced high dose model of asthma. ** p< 0.01 (Mann-Whitney t-test (a, b) or one-way ANOVA and Tukey's multiple comparison test (c, d)). Data are representative of 2 independent experiments with 5 mice per group (mean ± s.e.m. in a-d).

Supplementary Figure 7 Details of the transcriptomics analysis of AMs.

(a) Sequence and mapping statistics for raw Illumina data. (b) Validation of sample purity by assessing the expression of lineage-restricted marker genes for potential contaminants; eosinophils, neutrophils, B and T cells. (c) Unsupervised, hierarchical clustering of individual lanes demonstrating discrete clustering of biologic replicates. (d) Summary of differentially expressed (DE) genes (P<1e-5) in each pairwise comparison showing DE genes in blue in volcano plot, showing the total number of DE genes outside the bidirectional arrows, and showing in the arrowheads the direction of upregulated expression for all, moderately (log 2-fold change ± 2-4) and highly (log 2-fold change > 4) DE genes.

Supplementary Figure 8 Gating strategy for AM and MO subsets in BM, blood and BALF following infection with MuHV-4.

(a-c) Flow plots on MO subsets in BM, gated as liveCD19-CD11b+SiglecF-Ly6G-Ly6C+ cells (a), on MO subsets in blood gated as live CD11b+Ly6G-CD19-SiglecF-Ly6C+ cells (b), on AM defined as liveautofluorescent+CD11chi cells (c) and BALF MO gated as nonautofluorescentCD11cloCD19-CD11b+Ly6G-CCR2+Ly6C+ further analyzed for MHCII and Sca-1 expression (c). (d) Representative flow cytometry overlays of AM (as defined above) from BALB/c mock-infected mice (red) and AM (green) and MOs (blue) from MuHV-4 infected mice isolated from BALF at the different times p.i.. (e,f) Flow cytometry quantification of AM viability in BALF at different times p.i. using Annexin V-APC/7-AAD staining. Representative flow cytometry plots (e) and quantification of Annexin+/7AAD- and Annexin+/7AAD+ cells among AM (f) from mock or MuHV-4-infected BALB/c mice at different times p.i.. Data are mean ± s.e.m. of samples from 5 mice per group. *** p< 0.001, ** p< 0.01 and * p < 0.05 (in (f), all data were compared to values obtained at day 0 by one-way ANOVA and Dunnett's multiple comparison test).

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Machiels, B., Dourcy, M., Xiao, X. et al. A gammaherpesvirus provides protection against allergic asthma by inducing the replacement of resident alveolar macrophages with regulatory monocytes. Nat Immunol 18, 1310–1320 (2017). https://doi.org/10.1038/ni.3857

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