Supplementary Figure 4: Immune-cell composition and localization in TDLNs. | Nature Immunology

Supplementary Figure 4: Immune-cell composition and localization in TDLNs.

From: Tumor-induced stromal reprogramming drives lymph node transformation

Supplementary Figure 4

(a) T cell area (left) and B cell follicle size (right) as percentage/LN measured based on CD3εe+ or CD45R+ staining. (b) Flow cytometry of CD8α+ T cells (CD45+CD3ε+CD8α +) in NDLNs and day 11 TDLNs. (c) Confocal image of NDLN (top) and day 11 TDLN (bottom) stained for FoxP3 (green) CD3ε (red) and collagen I (blue). (d) Confocal image of NDLN (left), day 4 TDLN (middle) and day 11 TDLN (right) stained for CD3ε (green), CD45R (red) and collagen I (blue). (e) Confocal image of NDLN (top) and day 11 TDLN (bottom) of paracortical area (left) or B cell follicles (right) stained for EdU (green), CD45R (red), LYVE1 (blue), and CD3ε (magenta). (f) Flow cytometry quantification of CD45+EdU+ cells in NDLNs and day 11 TDLNs. Scale bars (c) 150 µm (d and e) 50 µm. Each symbol represents an individual LN (a,b,f). Small horizontal lines indicate mean ± s.e.m. *P <0.05, **P <0.01 (two-tailed unpaired t-test (a,f)). Data represent 3 independent experiments, n=6 NDLNs and n=5 TDLNs (a), n=6 NDLNs and n=6 TDLNs (b), n=8 NDLNs and n=12 TDLNs.

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