(a) Schematic illustration of the experimental protocol. (b) Flow cytometry of the frequencies of tdTomato+ IgG1+ class-switched plasmablasts/plasma cells (tdTomato+IgG1+B220loCD138hi) (upper panels), memory B cells (tdTomato+IgG1+B220hiCD138−CD38+) and GC B cells (tdTomato+IgG1+B220hiCD138−CD38−) (lower panels) among splenic NP-specific donor cells (CD45.2+NP+) in NP-OVA immunized CD45.1+ wild-type recipient mice given transferred with CD45.2+ S1pr2-ERT2cre-tdTomato B1-8ge naive cells. At day 9 post-immunization, the mice were treated with anti-DEC205-OVA or anti-DEC205-CS at the indicated doses and tamoxifen for 3 days before analysis. (c) Ratios of tdTomato+NP+IgG1+ memory B cells to tdTomato+NP+IgG1+ GC B cells (left) and tdTomato+NP+IgG1+ plasmablasts/plasma cells to tdTomato+NP+IgG1+ GC B cells (right) from Supplemental Fig. 7b. Each symbol (c) represents an individual mouse; small horizontal lines indicate the mean. Data are representative of two independent experiments (b, c; mean in c).