(a) Schematic illustration of the experimental protocol for Fig. 7. (b) qRT-PCR analysis of Bach2 mRNA to check the deletion efficiency of Bach2 haploid insufficient B cells. NP-specific IgG1+ donor GC B cells (NP+B220+IgG1+CD38−GL7+) were obtained from experiments in Fig. 7a. (c) Flow cytometry of EdU incorporated proliferating cells among splenic NP-specific IgG1+ plasmablasts/plasma cells (CD45.1+NP+B220loIgG1+CD138+), memory B cells (CD45.1+NP+B220+IgG1+CD138−CD38+) and GC B cells (CD45.1+NP+B220+IgG1+CD138−CD38−GL7+) in the NP-CGG immunized CD45.2+ wild-type recipient mice given transferred with CD45.1+ B1-8ge naïve B cells. At day10 post-immunization, the mice were treated with 1 mg EdU i.p. for 30 min before analysis (left). Bar graph showing the percentage of EdU+ cells in each population (right). Data are pooled from two independent experiments (b; mean and s.d. n=3 mice) and representative of two independent experiments (c; mean and s.d. n=3 mice).