Supplementary Figure 4: Effect of Bach2 deletion on the development of memory B cells and regulation of Bach2 expression. | Nature Immunology

Supplementary Figure 4: Effect of Bach2 deletion on the development of memory B cells and regulation of Bach2 expression.

From: Regulated selection of germinal-center cells into the memory B cell compartment

Supplementary Figure 4

(a) Schematic illustration of the experimental protocol for Fig. 4. (b) qRT-PCR analysis of Bach2 and Prdm1 mRNA in Bach2+/+Prdm1+/+ Mb1-cre, Bach2+/+Prdm1f/f Mb1-cre, or Bach2f/fPrdm1f/f Mb1-cre naive B cells to check the deletion efficiency for Fig. 5. (c) The graphs indicate the absolute numbers of NP-specific IgG1+ memory B cells (NP+B220+IgG1+CD38+) and GC B cells (NP+B220+IgG1+CD38) in the NP-CGG immunized Bach2+/+Prdm1+/+ Mb1-cre, Bach2+/+Prdm1f/f Mb1-cre or Bach2f/fPrdm1f/f Mb1-cre mice at day 28. (d, e, f) Schematic illustration of the experimental protocol for Fig. 5 (d), Fig. 6c (e), Fig. 6d (f), respectively. *p < 0.05 and **p < 0.01 (unpaired Student’s t-test). Each symbol (c) represents an individual mouse. Data are from one experiment (b; mean n=2 mice) and representative of three independent experiments (c; mean)

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