a) t-SNE plots based on surface marker intensities for 5 adult donors and 7 pediatric donors (n=20543) with intensities of the 4 markers used for t-SNE (NKp44, HLA-DR, CD62L and CD45RA) followed by donor distribution (red to yellow shades for pediatric, blue to green shades for adults). b) Bar charts show percentage of IL-2+, IL-22+, IL-17F+ and TNF+ cells from the indicated ILC3 subpopulations after IL-23 plus IL-1β (50 ng/ml each, for 12+6 hours) and/or PMA plus ionomycin (20 ng/ml plus 0.5 μM, for the last 6 hours). Bars show mean and SEM from 4-6 donors. **** p<0.0001, *** p<0.005, ** p<0.01 and * p<0.05 as calculated using oneway ANOVA and Tukey’s multi-comparisons test. c) Representative dot plots show intracellular IL-2 and IL-22 production by the different ILC3 subpopulations after the indicated stimulations.