Supplementary Figure 3: Pairwise comparisons of scRNA-seq profiles. | Nature Immunology

Supplementary Figure 3: Pairwise comparisons of scRNA-seq profiles.

From: The heterogeneity of human CD127+ innate lymphoid cells revealed by single-cell RNA sequencing

Supplementary Figure 3

a) Pairwise comparison of the mean expression of the top 50 differentially expressed transcripts for the two cell populations in question. Cells are colored according to cluster definition described in main Fig. 2. Cells where cluster definition and surface phenotype were in agreement are shown with a star (*). Cells that deviated in the clustering are either highlighted as cross (x) if they were defined as the other phenotype in the comparison, and as a circle (o) if the cell had another phenotype than the 2 cell populations in question. b) ERCC-ratio plots for each plot shown in a demonstrating the ratio of cellular RNA to ERCC spike-in RNA for each cell. Data were generated in three independent experiments with one tonsil donor each. Total number of cells per cell population: ILC1, n=112; ILC2, n=143; ILC3, n=320; NK cells, n=73.

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