Supplementary Figure 2 : SHP-2 plays a critical role in dectin-1- and C. albicans–induced gene expression.

From: Tyrosine phosphatase SHP-2 mediates C-type lectin receptor–induced activation of the kinase Syk and anti-fungal TH17 responses

Supplementary Figure 2

(a) BMDMs primed by IL-4 (10ng/ml) were stimulated by dectin-1 ligands Zymd (100μg/ml), ZymA (100μg/ml) or Curdlan (100μg/ml) for 24 h. Supernatants were collected for ELISA. (b) BMDCs were stimulated by Zymd (100μg/ml), MDP (10μg/ml), Pam3 (100ng/ml), CpGB (100nM), polyI:C (100μg/ml), or LPS (100ng/ml) for 24 h, and supernatants were collected for ELISA. (c) WT, FcRγ-KO and Mincle-KO BMDCs were stimulated with mannan (100μg/ml), TDB (50μg/well) or zymd (100μg/ml) for 24 h and secreted TNF was measured by ELISA. (d) BMDMs primed by IL-4 (10ng/ml) were stimulated by heat-killed yeast (MOI: 2) or hyphae of C. albicans (MOI: 1) for 24 h. Cytokines and chemokines were measured by ELISA. (e,f) BMDMs primed by GM-CSF (10ng/ml) were stimulated by dectin-2 ligand mannan (e) or Mincle ligand TDB (f) for 24 h. Supernatants were collected for ELISA. Data are presented as mean ± SEM from 3 samples for each group, and one representative experiment of three is presented. Note: * p<0.05, ** p<0.01, *** p<0.001.