Supplementary Figure 6 : Knocking down individual enzymes potentially involved in HPLA metabolism has no effect on nlp-29p::gfp expression.

From: Activation of a G protein–coupled receptor by its endogenous ligand triggers the innate immune response of Caenorhabditis elegans

Supplementary Figure 6

a, Part of the tyrosine metabolic pathway, showing the names and structures of intermediates and the names of selected candidate enzymes which potentially catalyze each step. To the best of our knowledge, how the keto of HPPA is converted into the hydroxy function of HPLA has not been elucidated in any eukaryotic species. b, Normalized fluorescence ratio of young adult IG1502 rde-1(ne219) V; Is[wrt-2p::rde-1; myo-2p::rfp]; frIs7 IV worms that are resistant to RNAi except in the epidermis (infected or not, left panel) or dpy-10 worms (right panel) carrying frIs7 treated with RNAi against control (sta-1, gfp and dcar-1) or candidate genes. Data are representative of at least two independent experiments with a minimum of 50 worms for each condition.