Supplementary Figure 4 : DHCA triggers PMK-1-dependent gene expression in the epidermis.

From: Activation of a G protein–coupled receptor by its endogenous ligand triggers the innate immune response of Caenorhabditis elegans

Supplementary Figure 4

Quantitative RT-PCR analysis of the expression of genes in the nlp-29 cluster in wild-type and pmk-1 worms treated with 5 mM DHCA for 2 h (a) or of the intestinal defence genes F49F1.6 and F57F4.4 (that require PMK-1 for their expression) and pgp-5, irg-1, irg-3 (that do not require PMK-1 for their expression)1,2 in wild-type and dcar-1(tm2484) worms after exposure to 5 mM DHCA for 2 h (b) or 5 h (c). Data are from three independent experiments (average and SD). The qualitative effect on nlp gene expression in the pmk-1 mutant mirrors that seen upon infection3, also with an apparent partial pmk-1-independent component for the induction of nlp-31 and nlp-34. Although exposure of worms to DHCA was sufficient to increase epidermal AMP gene expression, pre-treatment of worms with it did not provide any measurable protection against infection (results not shown).

1 - Estes, K. A., Dunbar, T. L., Powell, J. R., Ausubel, F. M. & Troemel, E. R. bZIP transcription factor zip-2 mediates an early response to Pseudomonas aeruginosa infection in Caenorhabditis elegans. Proc Natl Acad Sci U S A 107, 2153-2158 (2010).

2 - Dierking, K. et al. Unusual regulation of a STAT protein by an SLC6 family transporter in C. elegans epidermal innate immunity. Cell Host Microbe 9, 425-435 (2011).

3 - Pujol, N. et al. Distinct innate immune responses to infection and wounding in the C. elegans epidermis. Curr Biol 18, 481-489 (2008).