Supplementary Figure 3 : DCAR-1 acts in the epidermis to regulate nlp gene expression and resistance to infection with D. coniospora.

From: Activation of a G protein–coupled receptor by its endogenous ligand triggers the innate immune response of Caenorhabditis elegans

Supplementary Figure 3

a, Survival of wild-type, dcar-1(tm2484), dcar-1;dcar-1p::dcar-1::gfp, dcar-1;col-12p::dcar-1::gfp and sra-6p::dcar-1::Venus worms after infection with D. coniospora. For the experiment shown here, n= 70, 65, 67, 67 and 70 respectively). The statistical significance of the differences between strains is shown below the graph (one-sided log rank test). b, c Quantitative RT-PCR analysis of the expression of genes in the nlp-29 cluster in wild-type, dcar-1(tm2484) and dcar-1;dcar-1p::dcar-1::gfp (b) or wild-type, dcar-1(tm2484), dcar-1;col-12p::dcar-1 and dcar-1;sra-6p::dcar-1 worms (c) after infection with D. coniospora. Data are from three independent experiments (average and SD). d, Survival of wild-type, dcar-1(tm2484), dcar-1;col-12p::dcar-1 and with sra-6p::dcar-1 worms after infection with D. coniospora. For the experiment shown here, n= 62, 71, 55 and 65 respectively). The statistical significance of the difference between strains is shown below the graph (one-sided log rank test). The difference in survival between the strains common to the experiments shown in a and d is linked to a variation in pathogenicity between different preparations of fungal spores used in experiments performed on different days.