The blowout of the Macondo oil well in the Gulf of Mexico in April 2010 injected up to 500,000 tonnes of natural gas, mainly methane, into the deep sea1. Most of the methane released was thought to have been consumed by marine microbes between July and August 20102,3. Here, we report spatially extensive measurements of methane concentrations and oxidation rates in the nine months following the spill. We show that although gas-rich deepwater plumes were a short-lived feature, water column concentrations of methane remained above background levels throughout the rest of the year. Rates of microbial methane oxidation peaked in the deepwater plumes in May and early June, coincident with a rapid rise in the abundance of known and new methane-oxidizing microbes. At this time, rates of methane oxidation reached up to 5,900 nmol l−1 d−1—the highest rates documented in the global pelagic ocean before the blowout4. Rates of methane oxidation fell to less than 50 nmol l−1 d−1 in late June, and continued to decline throughout the remainder of the year. We suggest the precipitous drop in methane consumption in late June, despite the persistence of methane in the water column, underscores the important role that physiological and environmental factors play in constraining the activity of methane-oxidizing bacteria in the Gulf of Mexico.
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Joye, S. B., MacDonald, I. R., Leifer, I. & Asper, V. Magnitude and oxidation potential of hydrocarbon gases released from the BP oil well blowout. Nature Geosci. 4, 160–164 (2011).
Kessler, J. D. et al. A persistent oxygen anomaly reveals the fate of spilled methane in the deep Gulf of Mexico. Science 331, 312–315 (2011).
Valentine, D. L. et al. Dynamic autoinoculation and the microbial ecology of a deep water hydrocarbon irruption. Proc. Natl Acad. Sci. USA 109, 20286–20291 (2012).
De Angelis, M. A., Lilley, M. d., Olson, E. J. & Baross, J. A. Methane oxidation in deep-sea hydrothermal plumes of the endeavour segment of the Juan de Fuca Ridge. Deep-Sea Res. I 40, 1169–1186 (1993).
Valentine, D. L. et al. Propane respiration jump-starts microbial response to a deep oil spill. Science 330, 208–211 (2010).
Camilli, R. et al. Tracking hydrocarbon plume transport and biodegradation at Deepwater Horizon. Science 330, 201–204 (2010).
Bowles, M. W., Samarkin, V. A., Bowles, K. M. & Joye, S. B. Weak coupling between sulfate reduction and the anaerobic oxidation of methane in methane-rich seafloor sediments during ex situ incubation. Geochim. Cosmochim. Acta 75, 500–519 (2011).
Kunze, E. Near-inertial wave propagation in geostrophic shear. J. Phys. Oceanogr. 15, 544–565 (1985).
Polzin, K. L., Toole, J. M., Ledwell, J. R. & Schmitt, R. W. Spatial variability of turbulent mixing in the Abyssal Ocean. Science 276, 93–96 (1997).
Sassen, R. et al. Thermogenic gas hydrates and hydrocarbon gases in complex chemosynthetic communities, Gulf of Mexico continental slope. Org. Geochem. 30, 485–497 (1999).
Wankel, S. D. et al. New constraints on methane fluxes and rates of anaerobic methane oxidation in a Gulf of Mexico brine pool via in situ mass spectrometry. Deep-Sea Res. II 57, 2022–2029 (2010).
Reeburgh, W. S. Oceanic methane biogeochemistry. Chem. Rev. 107, 486–513 (2007).
Segers, R. Methane production and methane consumption: A review of processes underlying wetland methane fluxes. Biogeochemistry 41, 21–51 (1998).
Knief, C., Kolb, S., Bodelier, P. L., Lipski, A. & Dunfield, P. F. The active methanotrophic community in hydromorphic soils changes in response to changing methane concentration. Environ. Microbiol. 8, 321–333 (2006).
Tavormina, P. L., Ussler, W. III, Joye, S. B., Harrison, B. K. & Orphan, V. J. Distributions of putative aerobic methanotrophs in diverse pelagic marine environments. ISME J. 4, 1–11 (2010).
Carini, S., Bano, N., LeCleir, G. & Joye, S. B. Aerobic methane oxidation and methanotroph community composition during seasonal stratification in Mono Lake, California (USA). Environ. Microbiol. 7, 1127–1138 (2005).
Rivers, A. R. et al. Transcriptional response of bathypelagic marine bacterioplankton to the Deepwater Horizon oil spill. ISME J. 7, 2315–2329 (2013).
Tavormina, P. L., Ussler, W. III & Orphan, V. J. Planktonic and sediment-associated aerobic methanotrophs in two seep systems along the North American margin. Appl. Environ. Microbiol. 74, 3985–3995 (2008).
Murase, J. & Frenzel, P. Selective grazing of methanotrophs by protozoa in a rice field soil. Fems Microbiol. Ecol. 65, 408–414 (2008).
Bastviken, D., Ejlertsson, J., Sundh, I. & Tranvik, L. Methane as a source of carbon and energy for lake pelagic food webs. Ecology 84, 969–981 (2003).
Bouvier, T. & del Giorgio, P. A. Key role of selective viral-induced mortality in determining marine bacterial community composition. Environ. Microbiol. 9, 287–297 (2007).
Balasubramanian, R. et al. Oxidation of methane by a biological dicopper centre. Nature 456, 115–120 (2010).
Murrell, J. C., Gilbert, B. & McDonald, I. R. Molecular biology and regulation of methane monooxygenase. Arch. Microbiol. 173, 325–332 (2000).
Takeguchi, M., Ohashi, M. & Okura, I. Role of iron in particulate methane monooxygenase from Methylosinus trichosporium OB3b. Biometals 12, 123–129 (1999).
Bergquist, B. A. & Boyle, E. A. Dissolved iron in the tropical and subtropical Atlantic Ocean. Glob. Biogeochem. Cycles 20, GB1015 (2006).
Berson, O. & Lidstrom, M. E. Study of copper accumulation by the type I methanotroph Methylomicrobium albus BG8. Env. Sci. Tech. 30, 802–809 (1996).
Park, S., Shah, N. N., Taylor, R. T. & Droege, M. W. Batch cultivation of Methylosinus trichosporium OB3b I: Production of soluble methane monooxygenase. Biotechnol. Bioeng. 38, 423–433 (1991).
Joye, S. B., Bowles, M. W., Samarkin, V. A., Hunter, K. S. & Niemann, H. Biogeochemical signatures and microbial activity of different cold-seep habitats along the Gulf of Mexico deep slope. Deep-Sea Res. II 57, 1990–2001 (2010).
Mackelprang, R. et al. Metagenomic analysis of a permafrost microbial community reveals a rapid response to thaw. Nature 480, 368–371 (2011).
Sjostedt, J. et al. Recruitment of members from the rare biosphere of marine bacterioplankton communities after an environmental disturbance. Appl. Environ. Microbiol. 78, 1361–1369 (2012).
We thank C. Mann, A. Vossmeyer, J. Slaughter, C. Comerford, L. Potter, V. Samarkin and S. Cummings for assistance at sea and/or in the laboratory; M. Chistoserdova for providing advice on constructing qPCR primers and for providing pure cultures of methanotrophs; I. MacDonald, T. Treude and M. Chistoserdova provided constructive feedback on a previous version of this manuscript. Finally we thank the science parties and ship’s crews of RV Pelican, RV Nancy Foster, RV Walton Smith, RV Oceanus, RV Cape Hatteras, MY Arctic Sunrise and RV Atlantis. This work was supported by the NOAA Award NA07AR4300464 to the National Institute for Undersea Science and Technology (V.L.A., A.R.D. and S.B.J.), the Department of Energy (Gulf of Mexico Gas Hydrate Research Consortium, 07-11-036 to J.P.C.), the National Science Foundation (OCE-1043225 to S.B.J., OCE-0926699 to T.A.V. and OCE-1042934 to A.M.S.), the Gulf of Mexico Research Initiative (ECOGIG (S.B.J., V.L.A., A.B., J.P.C., A.R.D, J.P.M., C.D.M. and T.A.V.) and DEEP-C (J.P.C.)) and the Northern Gulf Institute (A.M.S.). Greenpeace and Texas A&M at Galveston facilitated the Arctic Sunrise expedition. This is ECOGIG contribution #192 and the data fall under GRIIDC accession number (R1.x132.134:0057).
The authors declare no competing financial interests.
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Crespo-Medina, M., Meile, C., Hunter, K. et al. The rise and fall of methanotrophy following a deepwater oil-well blowout. Nature Geosci 7, 423–427 (2014). https://doi.org/10.1038/ngeo2156
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