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Life in the hydrated suboceanic mantle


The recesses of the oceanic crust harbour microbes that influence geochemical fluxes between the solid Earth and the hydrosphere1,2. In the roots of the crust, mantle-derived rocks are progressively hydrated by hydrothermal circulation, a process known as serpentinization. The associated release of molecular hydrogen could provide metabolic energy for microbes3. Phylogenetic analyses of chimneys associated with seafloor hydrothermal systems have provided direct but spatially restricted evidence for the existence of active microbial communities in these hydrated rocks4; indirect evidence comes from isotopic analyses of drill cores5. Here, we examine fully serpentinized peridotites recovered from the Mid-Atlantic Ridge, using Raman microspectroscopy and electron microscopy. We detect high concentrations of organic matter, of two types, intimately associated with serpentine-hosted hydrogarnets. One type contains a complex mixture of aliphatic and aromatic compounds and functional groups such as amides, usually associated with biopolymers such as proteins, lipids and nucleic acids. The other corresponds to dense aggregates of thermally evolved carbonaceous matter, with a weak structural organization, which we attribute to the maturation of carbon compounds present in the other type of organic matter identified. We suggest that the observed endogenic accumulations of organic matter result from past microbial activity within the serpentinized oceanic crust, potentially supported by the by-products of serpentinization. We further suggest that the proposed crustal community mediates elemental fluxes from the Earth’s mantle to the oceans.

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Figure 1: C–Mg–Si mélange filling large cavities of a cracked H-Adr grain.
Figure 2: Association of DCM with H-Adr.
Figure 3: Association of organic polymers carrying biological functionalities with H-Adr.
Figure 4: Conceptual model from serpentinization to colonization.


  1. Santelli, C. M., Edgcomb, V. P., Bach, W. & Edwards, K. J. The diversity and abundance of bacteria inhabiting seafloor lavas positively correlate with rock alteration. Environ. Microbiol. 11, 86–98 (2009).

    Article  Google Scholar 

  2. Mason, O. U. et al. First investigation of the microbiology of the deepest layer of ocean crust. PLoS ONE 5, e15399 (2010).

    Article  Google Scholar 

  3. McCollom, T. M. Geochemical constraints on sources of metabolic energy for chemolithoautotrophy in ultramafic-hosted deep-sea hydrothermal systems. Astrobiology 7, 933–950 (2007).

    Article  Google Scholar 

  4. Kelley, D. S. et al. A serpentinite-hosted ecosystem: The Lost City hydrothermal field. Science 307, 1428–1434 (2005).

    Article  Google Scholar 

  5. Alt, J. C. & Shanks, W. C. III Sulfur in serpentinized oceanic peridotites: Serpentinization processes and microbial sulfate reduction. J. Geophys. Res. 103, 9917–9929 (1998).

    Article  Google Scholar 

  6. Peyve, A. A., Saveleva, G. N., Skolotnev, S. G. & Simonov, V. A. Tectonics and origin of the oceanic crust in the region of ‘dry’ spreading in the Central Atlantic (7 °10′–5°N). Geotectonics 37, 75–94 (2003).

    Google Scholar 

  7. Beard, J. S. & Hopkinson, L. A fossil, serpentinization-related hydrothermal vent, Ocean Drilling Program Leg 173, Site 1068 (Iberia Abyssal Plain): Some aspects of mineral and fluid chemistry. J. Geophys. Res. 105, 16527–16539 (2000).

    Article  Google Scholar 

  8. Spötl, C. W., Houseknecht, D. & Jaques, R. C. Kerogen maturation and incipient graphitization of hydrocarbon source rocks in the Arkoma Basin, Oklahoma and Arkansas: A combined petrographic and Raman spectrometric study. Org. Geochem. 28, 535–542 (1998).

    Article  Google Scholar 

  9. Maquelin, K. et al. Identification of medically relevant microorganisms by vibrational spectroscopy. J. Microbiol. Meth. 51, 255–271 (2002).

    Article  Google Scholar 

  10. Andreani, M., Grauby, O., Baronnet, A. & Munoz, M. Occurrence, composition and growth of polyhedral serpentine. Eur. J. Mineral. 20, 159–171 (2008).

    Article  Google Scholar 

  11. Kelemen, S. R. & Fang, H. L. Maturity trends in Raman spectra from kerogen and coal. Energ. Fuel. 15, 653–658 (2001).

    Article  Google Scholar 

  12. Waples, W. D. & Marzi, W. R. The universality of the relationship between vitrinite reflectance and transformation ratio. Org. Geochem. 28, 383–388 (1998).

    Article  Google Scholar 

  13. Fontaine, F. J., Cannat, M. & Escartin, J. Hydrothermal circulation at slow-spreading mid-ocean ridges: The role of along-axis variations in axial lithospheric thickness. Geology 36, 759–762 (2008).

    Article  Google Scholar 

  14. Cannat, M., Fontaine, F. J. & Escartı´n, J. in Diversity of Hydrothermal Systems on Slow Spreading Ocean Ridge (eds Rona, P., Devey, C., Dyment, J. & Murton, B.) (Geophysical Monograph Series Vol. 188, AGU, 2010).

    Google Scholar 

  15. McCollom, T. M. & Seewald, J. S. Abiotic synthesis of organic compounds in deep-sea hydrothermal environments. Chem. Rev. 107, 382–401 (2007).

    Article  Google Scholar 

  16. Proskurowski, G. et al. Abiogenic hydrocarbon production at Lost City hydrothermal field. Science 319, 604–607 (2008).

    Article  Google Scholar 

  17. Pasteris, J. D. Secondary graphitization in mantle-derived rocks. Geology 16, 804–807 (1988).

    Article  Google Scholar 

  18. Marshall, C. P., Edwards, H. G. M. & Jehlicka, J. Understanding the application of Raman spectroscopy to the detection of traces of life. Astrobiology 10, 229–243 (2010).

    Article  Google Scholar 

  19. Snow, J. E. & Dick, J. B. Pervasive magnesium loss by marine weathering of peridotite. Geochim. Cosmochim. Acta 59, 4219–4235 (1995).

    Article  Google Scholar 

  20. Chapelle, F. H. et al. A hydrogen-based subsurface microbial community dominated by methanogens. Nature 415, 312–315 (2002).

    Article  Google Scholar 

  21. Kashefi, K. & Lovley, D. R. Extending the upper temperature limit for life. Science 301, 934 (2003).

    Article  Google Scholar 

  22. Wankel, S. D. et al. Influence of subsurface biosphere on geochemical fluxes from diffuse hydrothermal fluids. Nature Geosci. 4, 461–468 (2011).

    Article  Google Scholar 

  23. Marcaillou, C., Muñoz, M., Vidal, O., Parra, T. & Harfouche, M. Mineralogical evidence for H2 degassing during serpentinization at 300 °C/300 bar. Earth Planet. Sci. Lett. 303, 281–290 (2011).

    Article  Google Scholar 

  24. Stupperich, E., Eisinger, H-J. & Schurr, S. Corrinoids in anaerobic bacteria. FEMS Microbiol. Lett. 87, 355–359 (1990).

    Article  Google Scholar 

  25. Florencio, L., Field, J. A. & Lettinga, G. Importance of cobalt for individual trophic groups in an anaerobic methanol-degrading consortium. Appl. Environ. Microbiol. 60, 227–234 (1994).

    Google Scholar 

  26. Delacour, A., Früh-Green, G. L., Bernasconi, S. M., Schaeffer, P. & Kelley, D. Carbon geochemistry of serpentines in the Lost City hydrothermal system. Geochim. Cosmochim. Acta 72, 3681–3702 (2008).

    Article  Google Scholar 

  27. Ehlmann, B. L., Mustard, J. F. & Murchie, S. L. Geologic setting of serpentine deposits on Mars. Geophys. Res. Lett. 37 (2010).

  28. Sleep, N. H., Meibom, A., Fridriksson, Th., Coleman, R. G. & Bird, D. K. H2-rich fluids from serpentinization: Geochemical and biotic implications. Proc. Natl Acad. Sci. USA 101, 12818–12823 (2004).

    Article  Google Scholar 

  29. Russell, M. J., Hall, A. J. & Martin, W. Serpentinization as a source of energy at the origin of life. Geobiology 8, 355–371 (2010).

    Article  Google Scholar 

  30. Ljungdahl, L. G. A life with acetogens, thermophiles, and cellulolytic anaerobes. Annu. Rev. Microbiol. 63, 1–25 (2009).

    Article  Google Scholar 

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We are grateful to A. Cipriani, who made available the sample collection recovered during the joint Russian–Italian S22 expedition from dredge site S2232, M. Ligi for providing the multibeam data and O. Boudouma for assistance during SEM. We thank F. Guyot, M. Andreani, A. Delacour, E. Galli, E. Passaglia, E. Gérard, M. van Zuilen and P. Philippot for discussion and support, along with N. H. Sleep for a constructive review. The authors have been funded by Fondazione Cassa di Risparmio di Modena through the CARBRIDGE project, the French CNRS-INSU INTERRVIE program (SERPECO project), an ECORD Research Grant 2010 to V.P. and the Région Ile de France (IPGP Raman facility). This is IPGP contribution 3241.

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All authors made the analyses, discussed the results and wrote the paper.

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Correspondence to Bénédicte Ménez or Daniele Brunelli.

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Ménez, B., Pasini, V. & Brunelli, D. Life in the hydrated suboceanic mantle. Nature Geosci 5, 133–137 (2012).

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