Abstract
It has recently been shown that RNA interference can be induced in cultured mammalian cells by delivery of short interfering RNAs (siRNAs). Here we describe a method for efficient in vivo delivery of siRNAs to organs of postnatal mice and demonstrate effective and specific inhibition of transgene expression in a variety of organs.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Fire,A. et al. Nature 391, 806–811 (1998).
Tuschl, T. Chembiochem 2, 239–245 (2001).
Billy, E., Brondani, V., Zhang, H., Muller, U. & Filipowicz, W. Proc. Natl Acad. Sci. USA 98, 14428–14433 (2001).
Svoboda, P., Stein, P., Hayashi, H. & Schultz, R.M. Development 127, 4147–4156 (2000).
Wianny, F. & Zernicka-Goetz, M. Nature Cell Biol. 2, 70–75 (2000).
Caplen, N.J., Fleenor, J., Fire, A. & Morgan, R.A. Gene 252, 95–105 (2000).
Elbashir, S.M. et al. Nature 411, 494–498 (2001).
Zamore, P.D., Tuschl, T., Sharp, P.A. & Bartel, D.P. Cell 101, 25–33 (2000).
Hammond, S.M., Bernstein, E., Beach, D. & Hannon, G.J. Nature 404, 293–296 (2000).
Parrish, S., Fleenor, J., Xu, S., Mello, C. & Fire, A. Mol. Cell 6, 1077–1087 (2000).
Elbashir, S.M., Lendeckel, W. & Tuschl, T. Genes Dev. 15, 188–200 (2001).
Caplen, N.J., Parrish, S., Imani, F., Fire, A. & Morgan, R.A. Proc. Natl Acad. Sci. USA 98, 9742–9747 (2001).
Zhang, G., Budker, V. & Wolff, J.A. Hum. Gene Ther. 10, 1735–1737 (1999).
Liu, F., Song, Y. & Liu, D. Gene Ther. 6, 1258–1266 (1999).
Okabe, M., Ikawa, M., Kominami, K., Nakanishi, T. & Nishimune, Y. FEBS Lett. 407, 313–319 (1997).
Acknowledgements
We thank J. Hegge, M. Noble, B. Hermanson, M. Finch, K. Vlotho and T. Reppen for technical assistance, and C. Wooddell for critically reading the manuscript.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
J.A.W. is Chief Scientific Officer and a member of the Board of Directors of Mirus Corporation.
Supplementary information
Rights and permissions
About this article
Cite this article
Lewis, D., Hagstrom, J., Loomis, A. et al. Efficient delivery of siRNA for inhibition of gene expression in postnatal mice. Nat Genet 32, 107–108 (2002). https://doi.org/10.1038/ng944
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/ng944
This article is cited by
-
Protein function annotation based on heterogeneous biological networks
BMC Bioinformatics (2022)
-
The role of non-coding RNAs in neuroprotection and angiogenesis following ischemic stroke
Metabolic Brain Disease (2020)
-
Adenovirus-mediated artificial MicroRNAs targeting matrix or nucleoprotein genes protect mice against lethal influenza virus challenge
Gene Therapy (2015)
-
PTEN–Foxo1 signaling triggers HMGB1-mediated innate immune responses in acute lung injury
Immunologic Research (2015)
-
Hemodynamics of a hydrodynamic injection
Molecular Therapy - Methods & Clinical Development (2014)
