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A gene-driven approach to the identification of ENU mutants in the mouse

Nature Genetics volume 30, pages 255256 (2002) | Download Citation

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Abstract

The construction of parallel archives of DNA and sperm from mice mutagenized with ethylnitrosurea (ENU) represents a potentially powerful and rapid approach for identifying point mutations in any gene in the mouse genome. We provide support for this approach and report the identification of mutations in the gene (Gjb2) encoding connexin 26, using archives established from the UK ENU mutagenesis program.

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Acknowledgements

This work was supported by the Medical Research Council, GlaxoSmithKline, Defeating Deafness, The European Community and the National Lottery Board. We thank Rachael Hardisty for helpful discussions.

Author information

Author notes

    • Emma L. Coghill
    •  & Alison Hugill

    These authors contributed equally to this work.

Affiliations

  1. MRC Mammalian Genetics Unit and UK Mouse Genome Centre, Harwell OX11 ORD, UK.

    • Emma L. Coghill
    • , Alison Hugill
    • , Nick Parkinson
    • , Claire Davison
    • , Peter Glenister
    • , Sian Clements
    • , Roger D. Cox
    •  & Steve D.M. Brown
  2. GlaxoSmithKline, New Frontiers Science Park, Harlow CM19 5AW, UK.

    • Jackie Hunter

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Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Steve D.M. Brown.

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DOI

https://doi.org/10.1038/ng847

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