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Assembly of microarrays for genome-wide measurement of DNA copy number


We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05–0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.

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Figure 1: Measurement of single-copy changes.
Figure 2: Genome-wide copy-number variation in two breast tumors.


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We thank J. Flint, D. Ledbetter, C. Lese and Vysis, Inc. for telomere clones and clones containing certain named genes. This work was supported by NIH grants CA80314, CA83040, CA84118, HD17665 and CA58207, by California BCRP grant 2RB-0225 and by Vysis, Inc.

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Correspondence to Donna G. Albertson.

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Snijders, A., Nowak, N., Segraves, R. et al. Assembly of microarrays for genome-wide measurement of DNA copy number. Nat Genet 29, 263–264 (2001).

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