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Assembly of microarrays for genome-wide measurement of DNA copy number

Abstract

We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05–0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.

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Figure 1: Measurement of single-copy changes.
Figure 2: Genome-wide copy-number variation in two breast tumors.

References

  1. 1

    Pinkel, D. et al. Nature Genet. 20, 207–211 (1998).

    CAS  Article  Google Scholar 

  2. 2

    Albertson, D.G. et al. Nature Genet. 25, 144–146 (2000).

    CAS  Article  Google Scholar 

  3. 3

    Klein, C.A., Schmidt-Kittler, O., Schardt, J.A., Pantel, K., Speicher, M.R. & Riethmuller, G. Proc. Natl Acad. Sci. USA 96, 4494–4499 (1999).

    CAS  Article  Google Scholar 

  4. 4

    Cheung, V.G. et al. Nature 409, 953–958 (2001).

    CAS  Article  Google Scholar 

  5. 5

    Knight, S.J.L. et al. Am. J. Hum. Genet. 67, 320–332 (2000).

    CAS  Article  Google Scholar 

  6. 6

    Geschwind, D.H. et al. Dev. Genet. 23, 215–29 (1998).

    CAS  Article  Google Scholar 

  7. 7

    Lucito, R. et al. Genome Res. 10, 1726–1736 (2000).

    CAS  Article  Google Scholar 

Download references

Acknowledgements

We thank J. Flint, D. Ledbetter, C. Lese and Vysis, Inc. for telomere clones and clones containing certain named genes. This work was supported by NIH grants CA80314, CA83040, CA84118, HD17665 and CA58207, by California BCRP grant 2RB-0225 and by Vysis, Inc.

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Correspondence to Donna G. Albertson.

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Snijders, A., Nowak, N., Segraves, R. et al. Assembly of microarrays for genome-wide measurement of DNA copy number. Nat Genet 29, 263–264 (2001). https://doi.org/10.1038/ng754

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