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Induction, suppression and requirement of RNA silencing pathways in virulent Agrobacterium tumefaciens infections

Nature Genetics volume 38, pages 258263 (2006) | Download Citation

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  • A Corrigendum to this article was published on 26 June 2015

Abstract

Regulation of gene expression through microRNAs (miRNAs) and antiviral defense through small interfering RNAs (siRNAs) are aspects of RNA silencing1, a process originally discovered as an unintended consequence of plant transformation by disarmed Agrobacterium tumefaciens strains2. Although RNA silencing protects cells against foreign genetic elements3, its defensive role against virulent, tumor-inducing bacteria has remained unexplored. Here, we show that siRNAs corresponding to transferred-DNA oncogenes initially accumulate in virulent A. tumefaciens–infected tissues and that RNA interference–deficient plants are hypersusceptible to the pathogen. Successful infection relies on a potent antisilencing state established in tumors whereby siRNA synthesis is specifically inhibited. This inhibition has only modest side effects on the miRNA pathway, shown here to be essential for disease development. The similarities and specificities of the A. tumefaciens RNA silencing interaction are discussed and contrasted with the situation encountered with plant viruses.

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Acknowledgements

We thank P. Zambryski and M. Matzke for critical reading of the manuscript, S. Gelvin for providing strains and the root inoculation protocol, members of the Voinnet lab for discussions and R. Wagner's team for plant care. This work was supported by an Action thématique et incitative sur programme Jeune Chercheur award from the Centre National de la Recherche Scientifique and a postdoctoral fellowship to P.D. from the Federation of European Biochemical Societies. O.V. is recipient of a Young Investigator Award from the European Molecular Biology Organization.

Author information

Affiliations

  1. CNRS UPR2357, IBMP, Strasbourg, France.

    • Patrice Dunoyer
    • , Christophe Himber
    •  & Olivier Voinnet

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Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Olivier Voinnet.

Supplementary information

PDF files

  1. 1.

    Supplementary Fig. 1

    A. thaliana plants expressing the P19 and P1-HcPro viral silencing suppressors show decreased susceptibility to A. tumefaciens.

  2. 2.

    Supplementary Fig. 2

    Absence of secondary siRNAs in stems and tumors from GF-RNAi plants with the rdr6 mutation.

  3. 3.

    Supplementary Fig. 3

    Optimal virus accumulation in stems of DAN2 plants carrying the rdr6 mutation, compared with tumors developing on similar DAN2 tissues.

  4. 4.

    Supplementary Fig. 4

    Specific transcriptional repression of miR393 in tumors.

  5. 5.

    Supplementary Table 1

    A. tumefaciens susceptibility assays in roots of the dcl2-1, dcl3-1 and rdr2-1 mutants.

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DOI

https://doi.org/10.1038/ng1722

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