Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

A screen of the complete protein kinase gene family identifies diverse patterns of somatic mutations in human breast cancer


We examined the coding sequence of 518 protein kinases, 1.3 Mb of DNA per sample, in 25 breast cancers. In many tumors, we detected no somatic mutations. But a few had numerous somatic mutations with distinctive patterns indicative of either a mutator phenotype or a past exposure.

This is a preview of subscription content

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Figure 1: Breast cancer mutational spectra.


  1. 1

    Manning, G., Whyte, D.B., Martinez, R., Hunter, T. & Sudarsanam, S. Science 298, 1912–1934 (2002).

    CAS  Article  Google Scholar 

  2. 2

    Huang, M.E., Rio, A.G., Nicolas, A. & Kolodner, R.D. Proc. Natl. Acad. Sci. USA. 100, 11529–11534 (2003).

    CAS  Article  Google Scholar 

  3. 3

    Xiao, W., Fontanie, T., Bawa, S. & Kohalmi, L. Mutat. Res. 431, 155–165 (1999).

    CAS  Article  Google Scholar 

  4. 4

    Wagner, E. & Lykke-Andersen, J. J. Cell Sci. 115, 3033–3038 (2002).

    CAS  PubMed  Google Scholar 

  5. 5

    Persons, D.L., Borelli, K.A. & Hsu, P.H. Mod. Pathol. 10, 720–727 (1997).

    CAS  PubMed  Google Scholar 

  6. 6

    Wang, T.L. et al. Proc. Natl. Acad. Sci. USA 99, 3076–3080 (2002).

    CAS  Article  Google Scholar 

  7. 7

    Olivier, M. et al. Hum. Mutat. 19, 607–614 (2002).

    CAS  Article  Google Scholar 

Download references

Author information



Corresponding author

Correspondence to Michael R Stratton.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Table 1

Mutation data for the primary and secondary screen (PDF 47 kb)

Supplementary Table 2

Genes analysed in the primary and secondary screens (XLS 66 kb)

Supplementary Table 3

Samples. (PDF 40 kb)

Supplementary Table 4

Coding strand. (PDF 52 kb)

Supplementary Table 5

Pyrimidine strand. (PDF 51 kb)

Supplementary Methods (PDF 78 kb)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Stephens, P., Edkins, S., Davies, H. et al. A screen of the complete protein kinase gene family identifies diverse patterns of somatic mutations in human breast cancer. Nat Genet 37, 590–592 (2005).

Download citation

Further reading


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing