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Sex, flies and microarrays

Nature Genetics volume 29, pages 355–356 (2001)Cite this article

In a typical microarray study, experimental RNA samples are compared to a reference sample and expression ratios are clustered. A new study of the effects of sex, aging and genotype on gene expression in Drosophila melanogaster departs from this paradigm. Highly replicated comparisons generate measures of expression that are analyzed using classical analysis of variance (ANOVA) techniques, revealing substantial differences in gene expression between the two sexes as well as smaller effects of age and strain. Moreover, this study demonstrates that replication and experimental design are essential to detecting small but biologically important differences in expression patterns.

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Figure 1: A 'volcano plot' reproduced from Jin et al.3
Figure 2: The pairings in a hypothetical microarray experiment using 32 microarrays to assay 16 samples are represented as a graph.

References

  1. Schena, M., Shalon, D., Davis, R.W. & Brown, P.O. Science 270, 467–470 (1995).

    Article  CAS  Google Scholar 

  2. Shalon, D., Smith, S.J. & Brown, P.O. Genome Res. 6, 639–645 (1996).

    Article  CAS  Google Scholar 

  3. Jin, W. et al. Nature Genet. 29, 389–395 (2001).

    Article  CAS  Google Scholar 

  4. Kerr, M.K. & Churchill, G.A. Genet. Res. Camb. 77, 123–128 (2001).

    CAS  Google Scholar 

  5. Fisher, R.A. The Design of Experiments 6th ed (Oliver and Boyd Edinburgh, 1951).

    Google Scholar 

  6. Kerr, M.K., Martin, M. & Churchill, G.A. J. Comp. Biol. 7, 819–837 (2000).

    Article  CAS  Google Scholar 

  7. Wolfinger, R. et al. J. Comp. Biol. (in the press).

  8. Tseng, G.C. et al. Nucleic Acids Res. 29, 2549–2557 (2001).

    Article  CAS  Google Scholar 

  9. Dudoit S. et al. Statistica Sinica (in the press).

  10. Kerr, M.K. & Churchill, G.A. Biostatistics 2, 183–201 (2001).

    Article  CAS  Google Scholar 

  11. Andrews, J. et al. G. Genome Res. 10, 2030–2043 (2000a).

    Article  CAS  Google Scholar 

  12. Reinke, V. et al. Mol. Cell 6, 605–616 (2000).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine, USA

    Gary A. Churchill

  2. Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, 50 South Drive, Bethesda, Maryland, USA

    Brian Oliver

Authors
  1. Gary A. Churchill
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  2. Brian Oliver
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Churchill, G., Oliver, B. Sex, flies and microarrays. Nat Genet 29, 355–356 (2001). https://doi.org/10.1038/ng1201-355

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