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The transcriptome of Arabidopsis thaliana during systemic acquired resistance

Abstract

Infected plants undergo transcriptional reprogramming during initiation of both local defence and systemic acquired resistance (SAR). We monitored gene-expression changes in Arabidopsis thaliana under 14 different SAR-inducing or SAR-repressing conditions using a DNA microarray representing approximately 25–30% of all A. thaliana genes. We derived groups of genes with common regulation patterns, or regulons. The regulon containing PR-1, a reliable marker gene for SAR in A. thaliana, contains known PR genes and novel genes likely to function during SAR and disease resistance. We identified a common promoter element in genes of this regulon that binds members of a plant-specific transcription factor family. Our results extend expression profiling to definition of regulatory networks and gene discovery in plants.

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Figure 1: Unrooted tree of relatedness of transcriptional changes across SAR-relevant conditions.
Figure 2: a, Clustergram of 413 ESTs differentially expressed during SAR.
Figure 3: Self-organizing map clusters of expression profiles.
Figure 4: W box motifs in PR-1 cluster genes.
Figure 5: Cis element architecture in the PR-1 cluster.

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Acknowledgements

We thank P. Rushton and I. Somssich for discussion; L. Friedrich, J. Levin and F. Zheng for RNA samples; A. Wiig for technical assistance; P. Epple for critical reading of the manuscript; the ABRC for EST clones; and S.Y. Rhee for calculation of average 5′ UTR lengths in A. thaliana genes. A.L. was supported by an REU supplement to NSF grant IBN-9724075 to J.L.D., and T.E. is the recipient of a Deutsche Forschungsgemeinschaft Post-doctoral Fellowship.

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Correspondence to Jeffery L. Dangl.

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Maleck, K., Levine, A., Eulgem, T. et al. The transcriptome of Arabidopsis thaliana during systemic acquired resistance. Nat Genet 26, 403–410 (2000). https://doi.org/10.1038/82521

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