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Rapid amplification of a retrotransposon subfamily is evolving the mouse genome

Nature Genetics volume 20, pages 288–290 (1998)Cite this article

Abstract

Retrotransposition affects genome structure by increasing repetition and producing insertional mutations1,2. Dispersion of the retrotransposon L1 throughout mammalian genomes suggests that L1 activity might be an important evolutionary force1. Here we report that L1 retrotransposition contributes to rapid genome evolution in the mouse, because a number of L1 sequences from the TF subfamily are retrotransposition competent. We show that the TF subfamily is large, young and expanding, containing approximately 4,800 full-length members in strain 129. Eleven randomly isolated, full-length TF elements averaged 99.8% sequence identity to each other, and seven of these retrotransposed in cultured cells. Thus, we estimate that the mouse genome contains approximately 3,000 active TF elements, 75 times the estimated number of active human L1s. Moreover, as TF elements are polymorphic among closely related mice, they have retrotransposed recently, implying rapid amplification of the subfamily to yield genomes with different patterns of interspersed repetition. Our data show that mice and humans differ considerably in the number of active L1s, and probably differ in the contribution of retrotransposition to ongoing sequence evolution.

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Figure 1: Eleven randomly cloned TF elements are nearly identical to each other.
Figure 2: TF elements are recent insertions.

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Acknowledgements

We thank K. Kaestner for providing the E14TG2a genomic library, C. Hutchison and M. Edgell for providing an A-monomer probe and D. Gasser, W. Silvers and B. Taylor for helpful discussions. We also thank J. Moran, E. Luning Prak and B. Dombroski for critical reading of the manuscript, and the DNA sequencing core at the University of Pennsylvania School of Medicine. H.H.K. was supported by an NIH grant.

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Authors and Affiliations

  1. Department of Genetics, University of Pennsylvania, Philadelphia, 19104, Pennsylvania, USA

    Ralph J. DeBerardinis, John L. Goodier, Eric M. Ostertag & Haig H. Kazazian Jr

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  1. Ralph J. DeBerardinis
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  2. John L. Goodier
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  4. Haig H. Kazazian Jr
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Correspondence to Haig H. Kazazian Jr.

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DeBerardinis, R., Goodier, J., Ostertag, E. et al. Rapid amplification of a retrotransposon subfamily is evolving the mouse genome. Nat Genet 20, 288–290 (1998). https://doi.org/10.1038/3104

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