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Deletion of Y chromosome sequences located outside the testis determining region can cause XY female sex reversal

Nature Genetics volume 5, pages 301–307 (1993)Cite this article

Abstract

An approach designed to map and generate mutations in the region of the short arm of the mouse Y chromosome, known to be involved in sex determination and spermatogenesis, is described. This relies on homologous Yp–Sxra pairing and asymmetrical exchange which can occur at meiosis in XY males carrying Sxra on their X chromosome. Such exchange potentially generates deficiencies and duplications of Yp or Sxra. Three fertile XY females were found out of about 450 XY offspring from XSxra/Y x XX crosses. In all three, despite evidence for deletion of Y chromosomal material, the Sry locus was intact. Each deletion involved a repeat sequence, Sx1, located at a distance from Sry. Since expression of Sry was affected these results suggest that long range position effects have disrupted Sry action.

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Authors and Affiliations

  1. Laboratory of Eukaryotic Molecular Genetics, MRC National Institute for Medical Research, The Ridgeway, Mill Hill London, NW7 1AA, UK

    Blanche Capel, Nigel Vivian & Robin Lovell-Badge

  2. Division of Genetics, MRC Radiobiology Unit, Chilton, Didcot, Oxon, 0X11 ORD, UK

    Carol Rasberry & Bruce M. Cattanach

  3. Section of Transplantation Biology, MRC Clinical Research Centre, Harrow, HA1 3UJ, UK

    Julian Dyson & Elizabeth Simpson

  4. Department of Obstetrics and Gynaecology, University of Tennessee, College of Medicine, 711 Jefferson Avenue, Memphis, Tennessee, 38103, USA

    Colin E. Bishop

  5. Section of Comparative Biology, MRC Clinical Research Centre, Harrow, HA1 3UJ, UK

    Sohaila Rastan

Authors
  1. Blanche Capel
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  2. Carol Rasberry
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  3. Julian Dyson
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  4. Colin E. Bishop
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  5. Elizabeth Simpson
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  6. Nigel Vivian
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  7. Robin Lovell-Badge
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  8. Sohaila Rastan
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  9. Bruce M. Cattanach
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Capel, B., Rasberry, C., Dyson, J. et al. Deletion of Y chromosome sequences located outside the testis determining region can cause XY female sex reversal. Nat Genet 5, 301–307 (1993). https://doi.org/10.1038/ng1193-301

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