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Human mitochondrial transcription factor B1 methylates ribosomal RNA at a conserved stem-loop

Nature Genetics volume 33, pages 2324 (2003) | Download Citation

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Abstract

Human mitochondrial transcription factor B1 (h-mtTFB1) has an unprecedented relationship to RNA methyltransferases. Here, we show that this protein methylates a conserved stem-loop in bacterial 16S rRNA and that the homologous sequence in the human mitochondrial 12S molecule is similarly modified. Thus, h-mtTFB1 appears to be dual-function protein, acting both as a transcription factor and an rRNA-modification enzyme.

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Acknowledgements

The authors thank A. Vila-Sanjurjo for supplying the ksgA bacterial strain. This work was supported by an R01 grant awarded to G.S.S. and an National Research Service Award to V.M. from the US National Institutes of Health.

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  1. Department of Biochemistry, Rollins Research Center, Emory University School of Medicine, Atlanta, Georgia 30322-3050, USA.

    • Bonnie L. Seidel-Rogol
    • , Vicki McCulloch
    •  & Gerald S. Shadel

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The authors declare no competing financial interests.

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Correspondence to Gerald S. Shadel.

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DOI

https://doi.org/10.1038/ng1064