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Human artificial episomal chromosomes for cloning large DNA fragments in human cells

Nature Genetics volume 8, pages 33–41 (1994)Cite this article

An Erratum to this article was published on 01 December 1994

Abstract

We have developed a human artificial episomal chromosome (HAEC) system, based on the latent replication origin of the large herpes Epstein-Barr virus, for the propagation and stable maintenance of DNA as circular minichromosomes in human cells. Individual HAECs carried human genomic inserts ranging from 60–330 kb and appeared genetically stable. An HAEC library of 1,500 independent clones carrying random human genomic fragments with average sizes of 150–200 kb was established and allowed recovery of the HAEC DNA. Our autologous HAEC system, with human DNA cloned directly in human cells, provides an important tool for functional study of large mammalian DNA regions and gene therapy.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, CB#;7295, 349 LCCC, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599, USA

    Tian-Qiang Sun & Jean-Michel H. Vos

  2. Curriculum in Genetics and Molecular Biology, CB#7295, 349 LCCC, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599, USA

    David A. Fenstermacher & Jean-Michel H. Vos

  3. UNC Lineberger Comprehensive Cancer Center, CB#7295, 349 LCCC, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599, USA

    Jean-Michel H. Vos

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Sun, TQ., Fenstermacher, D. & Vos, JM. Human artificial episomal chromosomes for cloning large DNA fragments in human cells. Nat Genet 8, 33–41 (1994). https://doi.org/10.1038/ng0994-33

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