Radiation hybrid map of the mouse genome

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Radiation hybrid (RH) maps are a useful tool for genome analysis, providing a direct method for localizing genes and anchoring physical maps and genomic sequence along chromosomes. The construction of a comprehensive RH map for the human genome1 has resulted in gene maps reflecting the location of more than 30,000 human genes2,3. Here we report the first comprehensive RH map of the mouse genome. The map contains 2,486 loci screened against an RH panel of 93 cell lines4. Most loci (93%) are simple sequence length polymorphisms (SSLPs) taken from the mouse genetic map, thereby providing direct integration between these two key maps. We performed RH mapping by a new and efficient approach in which we replaced traditional gel- or hybridization-based assays by a homogeneous 5´-nuclease assay5 involving a single common probe for all genetic markers. The map provides essentially complete connectivity and coverage across the genome, and good resolution for ordering loci, with 1 centiRay (cR) corresponding to an average of approximately 100 kb. The RH map, together with an accompanying World-Wide Web server, makes it possible for any investigator to rapidly localize sequences in the mouse genome. Together with the previously constructed genetic map6 and a YAC-based physical map reported in a companion paper7, the fundamental maps required for mouse genomics are now available.

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Figure 1: Scoring marker retention on the RH panel based on ratio of FAM to TAMRA fluorescence.
Figure 2: Integrated RH and genetic map of mouse chromosome 8.
Figure 3: Histogram of pair-wise lod scores between consecutive markers on the Whitehead Institute mouse RH reference map (white bars).


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We thank G. Farino and V. Frazzoni for technical assistance. T.J.H. is a recipient of a Clinician-Scientist award from the Medical Research Council of Canada. This research was supported by the National Institutes of Health (HG01806).

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Correspondence to Eric S. Lander or Thomas J. Hudson.

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Van Etten, W., Steen, R., Nguyen, H. et al. Radiation hybrid map of the mouse genome. Nat Genet 22, 384–387 (1999) doi:10.1038/11962

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