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Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library

Nature Genetics volume 4, pages 373–380 (1993)Cite this article

Abstract

A human infant brain cDNA library, made specifically for production of expressed sequence tags (ESTs) was evaluated by partial sequencing of over 1,600 clones. Advantages of this library, constructed for EST sequencing, include the use of directional cloning, size selection, very low numbers of mitochondrial and ribosomal transcripts, short polyA tails, few non-recombinants and a broad representation of transcripts. 37% of the clones were identified, based on matches to over 320 different genes in the public databases. Of these, two proteins similar to the Alzheimer's disease amyloid precursor protein were identified.

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Authors and Affiliations

  1. Receptor Biochemistry and Molecular Biology Section, NINDS/NIH, 9000 Rockville Pike, Bethesda, Maryland, 20892, USA

    Mark D. Adams, Anthony R. Kerlavage, Chris Fields & J. Craig Venter

  2. The Institute for Genomic Research, 932 Clopper Road, Gaithersburg, Maryland, 20878, USA

    Mark D. Adams, Anthony R. Kerlavage, Chris Fields & J. Craig Venter

  3. Department of Psychiatry, College of Physicians and Surgeons of Columbia University and New York State Psychiatric Institute, 722 W. 168th St., New York, New York, 10032, USA

    M. Bento Soares

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  1. Mark D. Adams
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  3. Anthony R. Kerlavage
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  4. Chris Fields
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  5. J. Craig Venter
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Adams, M., Soares, M., Kerlavage, A. et al. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat Genet 4, 373–380 (1993). https://doi.org/10.1038/ng0893-373

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