Human retina whole mounts were labelled with primary antibodies against ABCR and green/red cone opsin, blue cone opsin or rhodopsin and secondary antibodies conjugated with green or red dye. a, Merged confocal image of ABCR (Rim 5B4; green dye) and green/red cone opsin (red dye) labelling showing cones in yellow (merge of green and red) among the more abundant rods in green. b, Corresponding image showing only green/red cone opsin labelling in red. c, Conventional fluorescence image of ABCR (Rim 3F4) labelling of cones (arrows) surrounded by labelled rods. d, Rhodopsin (Rho 1D4) labelling of rods. e, Merged conventional fluorescence image of ABCR (Rim 3F4; green dye) and blue cone opsin (red dye) labelling showing blue cones in yellow (arrows) and rods in green. f, Corresponding image of blue cone opsin labelling in red. Insets are confocal images.