Genomic imprinting as originally described in Sciara1 is displayed by many organisms. In mammals, X-inactivation and the parent-of-origin-specific silencing of imprinted genes are examples of this phenomenon2,3. A heritable chromatin structural modification may be the critical mechanism in such instances of chromosome condensation and preferential gene inactivation. H19 is an imprinted gene4 in which the repressed paternal allele is hypermethylated and the compacted chromatin is relatively resistant to digestion by nucleases5–7. In order to uncover underlying conserved epigenetic mechanisms we have introduced a mouse H19 transgene into Drosophila. We show here that a 1.2-kb H19 upstream sequence functions in cis as a parent-of-origin independent silencing element in Drosophila. Strikingly, this cis-acting element is located within an upstream region that is necessary for H19 imprinting in mice8. These results suggest involvement of an evolutionary conserved mechanism in both gene silencing in Drosophila and imprinting in mice.
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