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p53 modulation of TFIIH–associated nucleotide excision repair activity

Nature Genetics volume 10, pages 188–195 (1995)Cite this article

Abstract

p53 has pleiotropic functions including control of genomic plasticity and integrity. Here we report that p53 can bind to several transcription factor IIH–associated factors, including transcription–repair factors, XPD (Rad3) and XPB, as well as CSB involved in strand–specific DNA repair, via its C–terminal domain. We also found that wild–type, but not Arg273His mutant p53 inhibits XPD (Rad3) and XPB DNA helicase activities. Moreover, repair of UV–induced dimers is slower in Li–Fraumeni syndrome cells (heterozygote p53 mutant) than in normal human cells. Our findings indicate that p53 may play a direct role in modulating nucleotide excision repair pathways.

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Authors and Affiliations

  1. Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20892-4255, USA

    X.W. Wang, H. Yeh, K. Forrester & C.C. Harris

  2. UPR 6520 (CNRS), Unite 184 (INSERM), Faculte de Medicine, 11 rue Humann, 67085, Strasbourg Cedex, France

    L. Schaeffer, R. Roy, V. Moncollin & J.-M. Egly

  3. Laboratory of Molecular Pathology, Department of Pathology, The University of Texas Southwestern Medical Center, Dallas, Texas, 75235, USA

    Z. Wang & E.C. Friedberg

  4. Laboratory of Molecular Genetics, National Institute of Aging, National Institutes of Health, Baltimore, Maryland, 21224, USA

    M.K. Evans, B.G. Taffe & V.A. Bohr

  5. Department of Cell Biology and Genetics, Medical Genetics Center, Erasmus University Rotterdam, P.O. Box 1738, 3000 DR, Rotterdam, The Netherlands

    G. Weeda & J.H.J. Hoeijmakers

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Wang, X., Yeh, H., Schaeffer, L. et al. p53 modulation of TFIIH–associated nucleotide excision repair activity. Nat Genet 10, 188–195 (1995). https://doi.org/10.1038/ng0695-188

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