Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

DNA double-strand breaks are not sufficient to initiate recruitment of TRF2

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Figure 1: TRF2 response to photoinduced DNA damage.
Figure 2: TRF2 fails to colocalize with DNA damage generated by α-particle irradiation.


  1. de Lange, T. Genes Dev. 19, 2100–2110 (2005).

    Article  CAS  Google Scholar 

  2. Karlseder, J. et al. PLoS Biol. 2, E240 (2004).

    Article  Google Scholar 

  3. Bradshaw, P.S., Stavropoulos, D.J. & Meyn, M.S. Nat. Genet. 37, 193–197 (2005).

    Article  CAS  Google Scholar 

  4. Limoli, C.L. & Ward, J.F. Radiat. Res. 134, 160–169 (1993).

    Article  CAS  Google Scholar 

  5. Rogakou, E.P., Boon, C., Redon, C. & Bonner, W.M. J. Cell Biol. 146, 905–916 (1999).

    Article  CAS  Google Scholar 

  6. Bedford, J.S. & Dewey, W.C. Radiat. Res. 158, 251–291 (2002).

    Article  CAS  Google Scholar 

  7. Aten, J.A. et al. Science 303, 92–95 (2004).

    Article  CAS  Google Scholar 

  8. Randers-Pehrson, G., Geard, C.R., Johnson, G., Elliston, C.D. & Brenner, D.J. Radiat. Res. 156, 210–214 (2001).

    Article  CAS  Google Scholar 

  9. Mone, M.J. et al. Proc. Natl. Acad. Sci. USA 101, 15933–15937 (2004).

    Article  CAS  Google Scholar 

  10. Bekker-Jensen, S. et al. J. Cell Biol. 173, 195–206 (2006).

    Article  CAS  Google Scholar 

Download references


We thank M. Cornforth for critical reading of the manuscript and for discussions. We also thank K. Mattern, D. van Gent and W. Vermeulen for providing cells; R.Y. Tsien for providing mCherry; C. Dinant for assistance with various laser-assisted local damaging techniques and C.H. van Oven and R.A. Hoebe for technical assistance. M.S.L. was supported by the Netherlands Organisation for Scientific Research (ZonMW 912-03-012). J.S., P.M.K. and J.A.A. were funded in part by a grant from the Dutch Cancer Society. E.S.W., R.L.U. and S.M.B. acknowledge support from the US National Institutes of Health (CA-09236 and CA-43322), US Department of Energy (DE-FG02-01ER63239) and the US National Aeronautics and Space Administration (NNJ04HD83G). The Radiological Research Accelerator Facility is supported through US National Institute of Biomedical Imaging and Bioengineering grant P41-EB002033.

Author information

Authors and Affiliations


Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Fig. 1

Recruitment of repair proteins in response to laser microirradiation. (PDF 501 kb)

Supplementary Fig. 2

TRF2 recruitment to microirradiated regions in the absence of Hoechst. (PDF 161 kb)

Supplementary Fig. 3

Quantification of fluorescence intensity of TRF2 after longitudinal exposure to one or two α-particles. (PDF 75 kb)

Supplementary Fig. 4

Protein response to localized exposure of 400 α-particles. (PDF 229 kb)

Supplementary Table 1

Summary of protein responses after exposure to various DNA damaging sources. (PDF 76 kb)

Supplementary Methods (PDF 147 kb)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Williams, E., Stap, J., Essers, J. et al. DNA double-strand breaks are not sufficient to initiate recruitment of TRF2. Nat Genet 39, 696–698 (2007).

Download citation

  • Issue Date:

  • DOI:

This article is cited by


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing