Quantitative mapping of amplicon structure by array CGH identifies CYP24 as a candidate oncogene

Abstract

We show here that quantitative measurement of DNA copy number across amplified regions using array comparative genomic hybridization1,2,3,4 (CGH) may facilitate oncogene identification by providing precise information on the locations of both amplicon boundaries and amplification maxima. Using this analytical capability, we resolved two regions of amplification within an approximately 2-Mb region of recurrent aberration at 20q13.2 in breast cancer. The putative oncogene ZNF217 (ref. 5) mapped to one peak, and CYP24 (encoding vitamin D 24 hydroxylase), whose overexpression is likely to lead to abrogation of growth control mediated by vitamin D (ref. 6), mapped to the other.

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Figure 1: Copy-number profiles for normal and tumour DNA samples on an array of clones from 20q13.2.
Figure 2: Relative expression of CYP24 in breast tumours.

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Acknowledgements

This work was supported by NIH grants CA80314, CA45919, HD17665 and P50 CA58207, California BCRP grant 2RB-0225 and Vysis.

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Correspondence to Donna G. Albertson.

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