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The imprinted antisense RNA at the Igf2r locus overlaps but does not imprint Mas1

Nature Geneticsvolume 25pages1921 (2000) | Download Citation

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Abstract

The gene encoding the insulin-like growth-factor type-2 receptor (Igf2r) is maternally expressed and imprinted1. A CpG island in Igf2r intron 2 that carries a maternal-specific methylation imprint2 was shown in a transgenic model to be essential for Igf2r imprinting and for the production of an antisense RNA from the paternal allele3. We report here that the endogenous region2 is the promoter for this antisense RNA (named Air, for antisense Igf2r RNA) and that the 3′ end lies 107,796 bp distant in an intron of the flanking, but non-imprinted4,5, gene Mas1.

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Acknowledgements

We thank R. Kurzbauer and S. Verhaagh for contributing sequences; E. Wagner for providing the region2 knockout mice; and A. Berns, J. Hewitt and F. Sleutels for critical reading of the manuscript. We acknowledge support from the Dutch Cancer Society (R.L.), The Institute of Molecular Pathology, Vienna, Austria (W.L., A.W.) and a Schrödinger Fellowship (O.W.S.).

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Author notes

    • Robert Lyle

    Present address: University of Geneva Medical School, Geneva, Switzerland

    • Christopher Davies

    Present address: Biotechnology Division, Bayer Corporation, Berkeley, California, USA

Affiliations

  1. The Netherlands Cancer Institute (H5), Amsterdam, The Netherlands

    • Robert Lyle
    • , Daisuke Watanabe
    • , Daniëlle te Vruchte
    • , Oskar W. Smrzka
    •  & Denise P. Barlow
  2. NIMR, The Ridgeway, London, UK

    • Walter Lerchner
  3. The Whitehead Institute, Cambridge, Massachusetts, USA

    • Anton Wutz
  4. University of Texas Southwestern Medical Center, Dallas, Texas, USA

    • Jeoffrey Schageman
    •  & Lisa Hahner

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Correspondence to Denise P. Barlow.

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https://doi.org/10.1038/75546

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