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Domain organization of allele–specific replication within the GABRB3 gene cluster requires a biparental 15q11–13 contribution

Nature Genetics volume 9, pages 386–394 (1995)Cite this article

Abstract

Imprinting marks the parental origin of chromosomes, resulting in allele–specific changes in chromatin organization, transcription and replication. We report a 50–60 kb domain of allele–specific replication between the γ–aminobutyric acid receptor subunit β3 (GABRB3) and α5 (GABRA5) genes. Replication of this domain occurs in early S phase on the maternal chromosome 15 but is delayed until the end of S phase on the paternal homologue. In contrast, the genomic regions flanking this domain exhibit paternal earlier replication in mid to late S phase. Uniparental disomy or hemizygous deletion of chromosome 15 results in altered allele–specific replication kinetics compared with normals, suggesting that allele–specific replication within the GABRB3/A5 region may be regulated by reciprocal imprints on the maternal and paternal chromosomes.

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Authors and Affiliations

  1. Howard Hughes Medical Institute,

    Janine M. LaSalle & Marc Lalande

  2. Genetics Division, Children's Hospital,

    Janine M. LaSalle & Marc Lalande

  3. Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, 02115

    Janine M. LaSalle & Marc Lalande

Authors
  1. Janine M. LaSalle
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  2. Marc Lalande
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LaSalle, J., Lalande, M. Domain organization of allele–specific replication within the GABRB3 gene cluster requires a biparental 15q11–13 contribution. Nat Genet 9, 386–394 (1995). https://doi.org/10.1038/ng0495-386

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