Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Monoallelic expression of the human H19 gene

Nature Genetics volume 1, pages 40–44 (1992)Cite this article

Abstract

Monoallelic expression of several genes has been observed in mice in which transcripts from parental homologues are distinguishable, but this phenomenon has not been demonstrated in humans. One monoallelically expressed murine gene, H19, encodes an abundant fetal RNA. We have found restriction site polymorphisms in the human H19 gene, located on chromosome 11p15, and examined the representation of these polymorphisms in cDNAs from fetal organs. Expression of H19 is largely or exclusively from a single allele; a similar analysis of the WT1 gene, on 11p13, shows biallelic expression. In the context of previous studies of 11p15 allelic losses in human embryonal tumours, our findings support the possibility of single-step inactivation of monoallelically expressed growth-regulating genes in human oncogenesis.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Swain, J.L., Stewart, T.A. & Leder, P. Cell 50, 719–727 (1987).

    Article  CAS  Google Scholar 

  2. DeChiara, T.M., Robertson, E.J. & Efstratiadis, A. Cell 64, 849–859 (1991).

    Article  CAS  Google Scholar 

  3. Barlow, D.P., Stoger, R., Herrmann, B.G., Saito, K. & Schweifer, N. Nature 349, 84–87 (1991).

    Article  CAS  Google Scholar 

  4. Bartolomei, M.S., Zemel, S. and Tilghman, S.M. Nature 351, 153–155 (1991).

    Article  CAS  Google Scholar 

  5. Pachnis, B., Belayew, A. and Tilghman, S.M. Proc. natn. Acad. Sci. U.S.A. 81, 5523–5527 (1984).

    Article  CAS  Google Scholar 

  6. Wiles, M.V. Development 104, 403–413 (1988).

    CAS  Google Scholar 

  7. Pachnis, V., Brannan, C.I. & Tilghman, S.M. EMBO J. 7, 673–681 (1988).

    Article  CAS  Google Scholar 

  8. Leibovitch, M.P. et al. Biochem. biophys. Res. Commun. 180, 1241–1250 (1991).

    Article  CAS  Google Scholar 

  9. Brannan, C.I., Clair Dees, E., Ingram, R.S. & tilghman, S.M. Molec. Cell Biol. 10, 28–36 (1990).

    Article  CAS  Google Scholar 

  10. Haber, D. and Housman, D.E., 64, 5–8 (1991).

  11. Pelletier, J. et al. Cell 67, 437–447 (1991).

    Article  CAS  Google Scholar 

  12. Hovan, P.R. & Kelsey, A.M. Nucl. Acids Res. 19, 1164 (1990).

    Google Scholar 

  13. Brunkow, M.E. & Tilghman, S.M. Genes Devel. 5, 1092–1101 (1991).

    Article  CAS  Google Scholar 

  14. Cattanach, B.M. & Kirk, M. Nature 315, 496–498 (1985).

    Article  CAS  Google Scholar 

  15. Sapienza, C., Tran, T-H., Paquette, J., McGowan, R. & Peterson, A. Prog. Nucl. Acids Res. Molec. Biol. 36, 145–157 (1989).

    Article  CAS  Google Scholar 

  16. Haig, D. & Graham, C. Cell 64, 1045–1046 (1991).

    Article  CAS  Google Scholar 

  17. Nicholls, R.D., Knoll, J.H.M., Butler, M.G., Karam, S. & Lalande, M. Nature 342, 281–285 (1989).

    Article  CAS  Google Scholar 

  18. Ridley, R.M., Frith, C.D., Crow, T.J. and Conneally, P.M. J. Med. Genet. 25, 589–595 (1988).

    Article  CAS  Google Scholar 

  19. Reik, W. J. med. Genet. 25, 805–808 (1988).

    Article  CAS  Google Scholar 

  20. Howeler, C.J. et al. Brain 112, 779–797 (1989).

    Article  Google Scholar 

  21. van der May, A.G.L. et al. Lancet ii, 1291–1294 (1989).

    Article  Google Scholar 

  22. Eldridge, R. Adv. Neurol. 29, 57–65 (1981).

    CAS  PubMed  Google Scholar 

  23. Schroeder, W.T. et al. Am. J. hum. Genet. 53, 89–140 (1987).

    Google Scholar 

  24. Pal, N. et al. Oncogene 5, 1665–1668 (1990).

    CAS  Google Scholar 

  25. Scrable, H. et al. Proc. natn. Acad. Sci. U.S.A. 86, 7480–7484 (1989).

    Article  CAS  Google Scholar 

  26. Henry, I. et al. Nature 351, 665–667 (1991).

    Article  CAS  Google Scholar 

  27. Koufos, A. et al. Am. J. hum. Genet. 44, 711–719 (1989).

    CAS  PubMed  PubMed Central  Google Scholar 

  28. Reeve, A.E., Sih, S.A., Raizis, A.M. & Feinberg, A.P. Molec. Cell. Biol. 9, 1799–1803 (1989).

    Article  CAS  Google Scholar 

  29. Glaser, T., Housman, D., Lewis, W.H., Gerhard, D. & Jones, C. Somatic Cell molec. Genet. 15, 477–501 (1989).

    Article  CAS  Google Scholar 

  30. Little, M., Van Heyningen, V. & Hastie, N. Nature 351, 609–610 (1991).

    Article  CAS  Google Scholar 

  31. Kittur, S.D. et al. Proc. natn. Acad. Sci. U.S.A. 82, 5064–5067 (1985).

    Article  CAS  Google Scholar 

  32. Searle, A.G. et al. Ann. hum. Genet. 53, 89–140 (1989).

    Article  CAS  Google Scholar 

  33. Maniatis, T., Fritsch, E.F. & Sambrook, J. in Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  34. Tycko, B., Palmer, J.D. & Sklar, J. Science 245, 1242–1246 (1989).

    Article  CAS  Google Scholar 

  35. Vogelstein, B. & Gillespie, D. Proc. natn. Acad. Sci. U.S.A. 76, 615–617 (1979).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Divisions of Oncology and Neuropathology, Department of Pathology, Columbia University College of Physicians and Surgeons, New York, New York, 10032, USA

    Yonghui Zhang & Benjamin Tycko

Authors
  1. Yonghui Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Benjamin Tycko
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, Y., Tycko, B. Monoallelic expression of the human H19 gene. Nat Genet 1, 40–44 (1992). https://doi.org/10.1038/ng0492-40

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/ng0492-40

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing