Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Automated DNA sequencing and analysis of 106 kilobases from human chromosome 19q13.3

Abstract

A total of 116, 118 basepairs (bp) derived from three cosmids spanning the ERCC1 locus of human chromosome 19q13.3 have been sequenced with automated fluorescence-based sequencers and analysed by polymerase chain reaction amplification and computer methods. The assembled sequence forms two contigs totalling 105,831 bp, which contain a human fosB proto-oncogene, a gene encoding a protein phosphatase, two genes of unknown function and the previously-characterized ERCC1 DNA repair gene. This light band region has a high average density of 1.4 Alu repeats per kilobase. Human chromosome light bands could therefore contain up to 75,000 genes and 1.5 million Alu repeats.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

References

  1. Van Duin, M. et al. Cell 44, 913–923 (1986).

    CAS  Article  Google Scholar 

  2. Van Duin, M. et al. Nucl. Acids Res. 15, 9195–9213 (1987).

    CAS  Article  Google Scholar 

  3. Smeets, H. et al. Am. J. hum.Genet. 46, 492–501 (1990).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Aslandis, C. et al. Nature 355, 548–551 (1992).

    Article  Google Scholar 

  5. Brook, J.D. et al. Cell 68, 799–808 (1992).

    CAS  Article  Google Scholar 

  6. De Jong, P.J. et al. Cytogenet. Cell Genet. 51, 985 (1989).

    Google Scholar 

  7. Carrano, A.V. et al. Genomics 4, 129–136 (1989).

    CAS  Article  Google Scholar 

  8. Carrano, A.V. et al. Genome 31, 1059–1065 (1989).

    CAS  Article  Google Scholar 

  9. Branscomb, E. et al. Genomics 8, 351–366 (1990).

    CAS  Article  Google Scholar 

  10. Schmid, C.W. & Jelinek, W.R. Science 216, 1065–1070 (1982).

    CAS  Article  Google Scholar 

  11. Fanning, T.G. & Singer, M.F. Biochem. biophys. Acta 910, 203–212 (1987).

    CAS  PubMed  Google Scholar 

  12. Paulson, K.E. et al. Nature 316, 359–361 (1985).

    CAS  Article  Google Scholar 

  13. Chen, E.Y. et al. Genomics 4, 479–497 (1989).

    CAS  Article  Google Scholar 

  14. Edwards, A. et al. Genomics 6, 593–608 (1990).

    CAS  Article  Google Scholar 

  15. Moyzis, R.K. et al. Genomics 4, 273–289 (1989).

    CAS  Article  Google Scholar 

  16. Korenberg, J.R. & M.C., Rykowski, Cell 53, 391–400 (1988).

    CAS  Article  Google Scholar 

  17. Bernardi, G. Ann. Rev. Genet. 23, 637–661 (1989).

    CAS  Article  Google Scholar 

  18. Slagel, V. et al. Molec. biol. Evol. 4, 19–29 (1987).

    CAS  PubMed  Google Scholar 

  19. Jurka, J. & Milosavljevic, A. J. molec. Evol. 32, 105–121 (1991).

    CAS  Article  Google Scholar 

  20. Matera, A., Hellman, U. & Schmid, C. Molec. cell. Biol. 10, 5424–5432 (1990).

    CAS  Article  Google Scholar 

  21. Batzer, M. & Deininger, P. Genomics 9, 481–487 (1991).

    CAS  Article  Google Scholar 

  22. Weber, J.L. Genomics 7, 524–530 (1990).

    CAS  Article  Google Scholar 

  23. Smeets, H.J.M. et al. Genomics 9, 257–263 (1991).

    CAS  Article  Google Scholar 

  24. Bird, A.P. Nature 321, 209–213 (1986).

    CAS  Article  Google Scholar 

  25. Uberbacher, E. & Mural, R. Proc. natn. Acad. Sci. U.S.A. 88, 11261–11265 (1991).

    CAS  Article  Google Scholar 

  26. Fields, C. & Soderlund, C. Comp. Appl. Biosci. 6, 261–270 (1990).

    Google Scholar 

  27. Soderlund, C. et al. Proc. Hawaii Int. Conf. Systems Sci. (IEEE Press) 653–662 (1992).

  28. Zerial, M. et al. EMBO J. 8, 805–813 (1989).

    CAS  Article  Google Scholar 

  29. Saunders, A. & Seldin, M. Genomics 6, 324–332 (1990).

    CAS  Article  Google Scholar 

  30. Lazo, P.S. et al. Nucl. Acids Res. 20, 343–350 (1992).

    CAS  Article  Google Scholar 

  31. Altschul, S. et al. J. molec. Biol. 215, 403–410 (1990).

    CAS  Article  Google Scholar 

  32. Tamura, S. et al. Proc. natn. Acad. Sci. U.S.A. 86, 1796–1800 (1989).

    CAS  Article  Google Scholar 

  33. Greenberg, M. & Ziff, E. Nature 311, 433–438 (1984).

    CAS  Article  Google Scholar 

  34. Muller, R. et al. Nature 312, 716–720 (1984).

    CAS  Article  Google Scholar 

  35. Molders, H. et al. Oncogene 1, 377–385 (1987).

    CAS  PubMed  Google Scholar 

  36. Nakabeppu, Y. & Nathans, D. Cell 64, 751–759 (1991).

    CAS  Article  Google Scholar 

  37. Mumberg, D. et al. Genes Dev. 5, 1212–1223 (1991).

    CAS  Article  Google Scholar 

  38. Roesler, W.J., Vandenbark, G.R. & Hanson, R.W. J. biol. Chem. 263, 9063–9066 (1988).

    CAS  PubMed  Google Scholar 

  39. Quinn, P.G. et al. Molec. cell. Biol. 8, 3467–3475 (1988).

    CAS  Article  Google Scholar 

  40. Lemaire, P. et al. Molec. cell. Biol. 10, 3456–3467 (1990).

    CAS  Article  Google Scholar 

  41. Fichant, G.A. & Burks, C. J. molec. Biol. 220, 659–671 (1991).

    CAS  Article  Google Scholar 

  42. Van Duin, M. et al. Molec. cell. Biol. 9, 1794–1798 (1989).

    CAS  Article  Google Scholar 

  43. Adams, M.D. et al. Science 252, 1651–1656 (1991).

    CAS  Article  Google Scholar 

  44. Adams, M.D. et al. Nature 355, 632–634 (1992).

    CAS  Article  Google Scholar 

  45. Barnes, D.E. et al. Genomics (in the press).

  46. Sambrook, J.E., & Maniatis, T. Molecular Cloning: A Laboratory Manual. 3rd edn (Cold Spring Harbor Press, 1989).

    Google Scholar 

  47. McCombie, W.R. et al. DNA Sequence (in the press).

  48. Sanger, F., Nicklen, S. & Coulson, A. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    CAS  Article  Google Scholar 

  49. Dubnick, M., Comp. appl. Biosci. (in the press).

  50. McCombie, W.R. et al. Methods 3, 33–40 (1991).

    CAS  Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Martin-Gallardo, A., McCombie, W., Gocayne, J. et al. Automated DNA sequencing and analysis of 106 kilobases from human chromosome 19q13.3. Nat Genet 1, 34–39 (1992). https://doi.org/10.1038/ng0492-34

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/ng0492-34

Further reading

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing