Abstract
The strategy presented here to identify unequivocally cryptic chromosomal rearrangements has relevance to both prenatal and postnatal cytogenetic analysis as well as the analysis of tumour-associated chromosome rearrangements. Microdissection and in vitro amplification of specific chromosomal regions are performed, followed by labelling for fluorescent in situ hybridization (FISH) to normal metaphase chromosomes (Micro-FISH). Micro-FISH probes have been used successfully to determine the derivation of chromosome segments unidentifiable by standard chromosome banding analysis. Micro-FISH probes (created in less than 24 hours) now make it possible to identify explicitly the chromosome constitution of virtually all cytologically visible chromosome rearrangements.
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References
Hassold, T. Trends Genet. 2, 105–110 (1986).
de Grouchy, J. & Turleau, C. Clinical Atlas of Human Chromosomes 2nd edn (John Wiley & Sons, New York, 1984).
Gardner, R. & Sutherland, G. Chromosome Abnormalities and Genetic Counseling (Oxford University. Press, New York, 1989).
Gelehrter, T. & Collins, F. Principals of Medical Genetics 159–189 (Williams & Wilkins, Baltimore, MD, 1990).
Heim, S. & Mitelman, F. Cancer Cytogenetics (Liss, New York, 1987).
Sandberg, A. The Chromosomes in Human Cancer and Leukemia (Elsevier, New York, 1990).
Trent, J. et al. New Engl. J. Med 322, 1508–1511 (1990).
Haluska, F., Trujimoto, Y. & Croce, C. Ann. Rev. Genet. 21, 321–345 (1987).
Weinberg, R. J. NIH Res. 3, 45–49 (1991).
Nowell, P. & Croce, C. Am. J. clin. Pathol. 94, 229–237 (1990).
Rowley, J. Cancer Res. 50, 3816–3825 (1990).
Fearon, E. & Vogelstein, B. Cell 61, 759–767 (1990).
Fountain, J. Science 244, 1085–1087 (1989).
Warburton, D. Am. J. hum. Genet. 49, 995–1013 (1991).
Callen, D. et al. Am. J. hum. Genet. 48, 769–782 (1991).
Mitelman, F. Catalog of Chromosome Aberrations in Cancer 4th edn (Wiley-Liss, New York, 1991).
Mitelman, F., Kaneko, Y. & Trent, J. Cytogenet. Cell Genet. 58, 1053–1079 (1991).
Pinkel, D. et al. Proc. natn. Acad. Sci. U.S.A. 85, 9138–42 (1988).
Lichter, P. et al. Hum. Genet. 80, 224–234 (1988).
Kuo, W-L. et al. Am. J. hum. Genet. 49, 112–119 (1991).
Meese, E. et al. Genomics 12, 542–548 (1992).
Tkachuk, D. et al. Science 250, 559–562 (1990).
Kuwano, A. et al. Am. J. hum. Genet. 49, 707–714 (1991).
Johnson, D. Genomics 6, 243–251 (1990).
Trautmann, U., Leutritz, G. & Senger, U. et al. Hum. Genet. 87, 495–497 (1991).
Lengauer, C. et al. Cytogenet. Cell Genet. 56, 27–30 (1991).
Hampton, D. et al. Genomics 11, 247–251 (1991).
Trent, J., Rosenfeld, S. & Meyskens, F. Cancer genet. Cytogenet. 9, 177–180 (1983).
Pathak, S., Drwinga, H. & Hsu, T-C. Cytogenet. Cell Genet. 36, 573–579 (1983).
Trent, J. et al. Science 247, 568–571 (1990).
Millikin, D. et al. Cancer Res. 51, 5449–5453 (1991).
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Meltzer, P., Guan, XY., Burgess, A. et al. Rapid generation of region specific probes by chromosome microdissection and their application. Nat Genet 1, 24–28 (1992). https://doi.org/10.1038/ng0492-24
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DOI: https://doi.org/10.1038/ng0492-24
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