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Identification of the remains of the Romanov family by DNA analysis


Nine skeletons found in a shallow grave in Ekaterinburg, Russia, in July 1991, were tentatively identified by Russian forensic authorities as the remains of the last Tsar, Tsarina, three of their five children, the Royal Physician and three servants. We have performed DNA based sex testing and short tandem repeat (STR) analysis and confirm that a family group was present in the grave. Analysis of mitochondrial (mt) DNA reveals an exact sequence match between the putative Tsarina and the three children with a living maternal relative. Amplified mtDNA extracted from the remains of the putative Tsar has been cloned to demonstrate heteroplasmy at a single base within the mtDNA control region. One of these sequences matches two living maternal relatives of the Tsar. We conclude that the DNA evidence supports the hypothesis that the remains are those of the Romanov family.

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  1. Fregeau, C.J. & Fourney, R.M. DNA typing with a fluorescently-tagged short tandem repeats: A sensitive and accurate approach to human identification. Biotechniques 15, 100–119 (1993).

    CAS  PubMed  Google Scholar 

  2. Kimpton, C.P. et al. Automated DNA profiling employing ‘multiplex’ amplification of short tandem repeat loci. PCR Meth. Applic. 13, 13–22 (1993).

    Article  Google Scholar 

  3. Hagelberg, E., Gray, I.C. & Jeffreys, A.J. Identification of the skeletal remains of a murder victim by DNA analysis. Nature 352, 427–429 (1991).

    Article  CAS  Google Scholar 

  4. Jeffreys, A.J., Allen, M.J., Hagelberg, E. & Sonnberg, A. Identification of the skeletal remains of Josef Mengele by DNA analysis. For. Sci. Int. 56, 65–76 (1992).

    CAS  Google Scholar 

  5. Bogenhagen, D. & Clayton, D.A. The number of mitochondrial deoxyribonucleic acid genomes in mouse L and human HeLa cells. J. biol. Chem. 249, 7991–7995 (1974).

    CAS  PubMed  Google Scholar 

  6. Paabo, S., Gifford, J.A. & Wilson, A.C. Mitochondrial DNA sequences from 7000 year old brain. Nucl. Acids Res. 16, 9775–9785 (1988).

    Article  CAS  Google Scholar 

  7. Hagelberg, E., Sykes, B. & Hedges, R. Ancient bone DNA amplified. Nature 342, 485 (1989).

    Article  CAS  Google Scholar 

  8. Sullivan, K.M., Mannucci, A., Kimpton, C. & Gill, P. A Rapid and Quantitative DNA Sex Test: Fluorescence-based PCR analysis of X-Y homologous gene amelogenin. Biotechniques (in the press).

  9. Mannucci, A., Sullivan, K.M., Ivanov, P.L. & Gill, P. Forensic application of a rapid and quantitative DNA sex text by amplification of the X-Y homologous gene amelogenin. Int. J. leg. Med. 15, 636–641 (1993).

    Google Scholar 

  10. Edwards, A., Civitello, A., Hammond, H.A. & Caskey, C.T. DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am J. hum. Genet. 49, 746–756. (1991).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Kimpton, C.P., Walton, A. & Gill, P. A further tetranucleotide repeat polymorphism in the vWF gene. Hum. molec. Genet. 1, 287 (1992).

    Article  CAS  Google Scholar 

  12. Polymeropoulos, M.H., Rath, D.S., Xiao, H. & Merril, C.R. Tetranuoleotide repeat polymorphism at the human coagulation factor XIII A subunit gene (F13A1). Nucl. Acids Res. 19, 4036 (1991).

    Google Scholar 

  13. Polymeropoulos, M.H., Rath, D.S., Xiao, H. & Merril, C.R. Tetranucleotide repeat polymorphism at the human c-fes/fps proto-oncogene (FES). Nucl. Acids Res. 19, 4791 (1991).

    Google Scholar 

  14. Warne, D., Warkins, P., Bodfish, P., Nyberg, K. & Spurr, N.K. Tetranucleotide repeat polymorphism atthehuman betaactin related pseudogene2 (ACTBP2) detected using the polymerase chain reaction. Nucl. Acids Res. 19, 6980 (1991).

    CAS  PubMed  Google Scholar 

  15. Howell, N. et al. Mitochondrial gene segregation in mammals: is the bottleneck always narrow? Hum. Genet. 90, 117–120 (1992).

    Article  CAS  Google Scholar 

  16. Koehler, C.M. et al. Replacement of bovine mitochondrial DNA by a sequence variant within one generation. Genetics 129, 247–255 (1991).

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Piercy, R., Sullivan, K.M., Benson, N. & Gill, P. The application of mitochondrial DNA typing to the study of white Caucasian identification. Int. J. leg. Med. 106, 85–90 (1993).

    Article  CAS  Google Scholar 

  18. Hagelberg, E. & Clegg, J. Isolation and characterisation of DNA from archeaological bone. Proc. R. Soc. Lond. B. 244, 45–50 (1991).

    Article  CAS  Google Scholar 

  19. Sullivan, K.M., Hopgood, R., Lang, B. & Gill, P. Automated amplification and sequencing of human mitochondrial DNA. lectrophoresis 12, 17–21 (1991).

    Article  CAS  Google Scholar 

  20. Orrego, C. & King, M.-C. Determination of familial relationships. In PCR protocols(eds M.A. Innis, D.H. Gelfand, J.J. Sninsky and T.J. White) 416–426 (Academic Press, London, 1990).

    Google Scholar 

  21. Anderson, S. et al. Sequence and organization of the human mitochondrial genome. Nature 290, 457–465 (1981).

    Article  CAS  Google Scholar 

  22. Maniatis, T., Fritsch, E.F. & Sambrook, J. Molecular cloning: a laboratory manual. (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  23. Stoneking, M., Sherry, S.T., Redd, A.J. & Vigilant, L. New approaches to dating suggest a recent age for the human mtDNA ancestor. Phil. Trans. R. Soc. Lond. B. 337, 167–175 (1992).

    Article  CAS  Google Scholar 

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Gill, P., Ivanov, P., Kimpton, C. et al. Identification of the remains of the Romanov family by DNA analysis. Nat Genet 6, 130–135 (1994).

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