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Expression profiling using cDNA microarrays

Nature Genetics volume 21, pages 10–14 (1999)Cite this article

Abstract

cDNA microarrays are capable of profiling gene expression patterns of tens of thousands of genes in a single experiment. DNA targets, in the form of 3´ expressed sequence tags (ESTs), are arrayed onto glass slides (or membranes) and probed with fluorescent– or radioactively–labelled cDNAs. Here, we review technical aspects of cDNA microarrays, including the general principles, fabrication of the arrays, target labelling, image analysis and data extraction, management and mining.

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Figure 1: cDNA microarray schema.
Figure 2: Quantitations from two–colour hybridization.
Figure 3: Atomic force microscopy of DNA on a microarray.
Figure 4: Detection schemes and applications of cDNA microarrays.

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Acknowledgements

A host of talented investigators have contributed to the NIH Microarray Project, including: Y. Jiang, A. Glatfelter, G. Gooden, J. Kahn, M. Boguski, G. Schuler, O. Ermolaeva, E. Dougherty, T. Pohida, P. Smith, S. Leighton, J. Hudson, A. Fornace, S. Amundson, S. Zeichner, C. Xiang, R. Simon, J. DeRisi & P. Brown.

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Authors and Affiliations

  1. Cancer Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, 20892, Maryland, USA

    David J Duggan, Michael Bittner, Yidong Chen, Paul Meltzer & Jeffrey M. Trent

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  1. David J Duggan
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Duggan, D., Bittner, M., Chen, Y. et al. Expression profiling using cDNA microarrays. Nat Genet 21 (Suppl 1), 10–14 (1999). https://doi.org/10.1038/4434

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