Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

Generalized lacZ expression with the ROSA26 Cre reporter strain

Nature Genetics volume 21, pages 70–71 (1999)Cite this article

Mouse strains expressing the site-specific recombinase Cre (or Flp) facilitate conditional ablation of gene function when one or several exons of the gene of interest are flanked by loxP (or FRT) sites1. Cre expression achieved by classic transgenesis or targeting to an appropriate locus might be tissue specific, temporally restricted or inducible2,3. In such experimental outlines, it is necessary to monitor Cre activity at desired time points as well as to verify that Cre was not active previously during development. Other investigators have generated transgenic4,5 or knock-in6 lines in which lacZ expression is conditional on the removal of an intervening segment. However, such lines are most useful if lacZ can be expressed in all cell types and hence is driven off a constitutively active promoter in the mouse.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: ROSA 26 targeting.
Figure 2: LacZ expression following Cre recombination

References

  1. Rajewsky, K. et al. J. Clin. Invest. 98, 600– 603 (1996).

    Article  CAS  Google Scholar 

  2. Feil, R. et al. Proc. Natl Acad. Sci. USA 93, 10887– 10890 (1996).

    Article  CAS  Google Scholar 

  3. Kellendonk, C. et al. Nucleic Acids Res. 24, 1404– 1411 (1996).

    Article  CAS  Google Scholar 

  4. Akagi, K. et al. Nucleic Acids Res. 25, 1766– 1773 (1997).

    Article  CAS  Google Scholar 

  5. Tsien, J.Z. et al. Cell 87, 1317–1326 (1996).

    Article  CAS  Google Scholar 

  6. O'Gorman, S., Dagenais, N.A., Qian, M. & Marchuk, Y. Proc. Natl Acad. Sci. USA 94, 14602–14607 (1997).

    Article  CAS  Google Scholar 

  7. Friedrich, G.A. & Soriano, P. Genes Dev. 5, 1513–1523 (1991).

    Article  CAS  Google Scholar 

  8. Zambrowicz, B.P. et al. Proc. Natl Acad. Sci. USA 94, 3789 –3794 (1997).

    Article  CAS  Google Scholar 

  9. Maxwell, I.H., Harrison, G.S., Wood, W.M. & Maxwell, F. BioTechniques 7, 276–280 (1989).

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

I thank C. Auger, P. Capp and K. Weismann for technical assistance. This work was supported by NIH grant RO1 HD 24875.

Author information

Authors and Affiliations

  1. Division of Basic Sciences, Program in Developmental Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North , Seattle, 98109, Washington, USA

    Philippe Soriano

Authors
  1. Philippe Soriano
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Soriano, P. Generalized lacZ expression with the ROSA26 Cre reporter strain . Nat Genet 21, 70–71 (1999). https://doi.org/10.1038/5007

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/5007

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing