A peptide needle in a signaling haystack

Expressing random peptides within cells has been hampered by technical problems, and so a simple method to express random free peptide libraries within mammalian cells comes as a welcome advance. It offers a powerful new way to approach the analysis of complex signal transduction cascades, as is illustrated in dissecting the emergence of tumor cell resistance to the chemotherapeutic drug taxol.

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  1. 1

    Xu, X. et al. Nature Genet. 27, 23–29 (2001).

  2. 2

    Blum, J.H., Dove, S.L., Hochschild, A. & Mekalanos, J.J. Proc. Natl. Acad. Sci. USA 97, 2241– 2246 (2000).

  3. 3

    Bodenmuller, H., Schilling, E., Zachmann, B. & Schaller, H. EMBO J. 5, 1825–1829 ( 1986).

  4. 4

    Chen, C.-j. et al. Cell 47, 381–389 (1986).

  5. 5

    Wei, L.Y. & Roepe, P.D. Biochemistry 33, 7229–7238 (1994).

  6. 6

    Hoffman, M.M., Wei, L.-Y. & Roepe, P.D. J. Gen. Physiol. 108, 295– 313 (1996).

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Roepe, P. A peptide needle in a signaling haystack. Nat Genet 27, 6–8 (2001) doi:10.1038/83775

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