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Hacking DNA copy number for circuit engineering

Nature Genetics volume 49, pages 1164–1165 (2017)Cite this article

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DNA copy number represents an essential parameter in the dynamics of synthetic gene circuits but typically is not explicitly considered. A new study demonstrates how dynamic control of DNA copy number can serve as an effective strategy to program robust oscillations in gene expression circuits.

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Figure 1: Dynamic DNA copy number control for programming cellular functions.

References

  1. Sebat, J. et al. Science 305, 525–528 (2004).

    Article  CAS  Google Scholar 

  2. Gresham, D. et al. Proc. Natl. Acad. Sci. USA 107, 18551–18556 (2010).

    Article  CAS  Google Scholar 

  3. Narula, J. et al. Cell 162, 328–337 (2015).

    Article  CAS  Google Scholar 

  4. Elowitz, M.B., Levine, A.J., Siggia, E.D. & Swain, P.S. Science 297, 1183–1186 (2002).

    Article  CAS  Google Scholar 

  5. Korem, T. et al. Science 349, 1101–1106 (2015).

    Article  CAS  Google Scholar 

  6. Baumgart, L., Mather, W. & Hasty, J. Nat. Genet. 49, 1282–1285 (2017).

    Article  CAS  Google Scholar 

  7. Friehs, K. Adv. Biochem. Eng. Biotechnol. 86, 47–82 (2004).

    CAS  PubMed  Google Scholar 

  8. Cameron, D.E., Bashor, C.J. & Collins, J.J. Nat. Rev. Microbiol. 12, 381–390 (2014).

    Article  CAS  Google Scholar 

  9. Cao, Y., Lopatkin, A. & You, L. Nat. Struct. Mol. Biol. 23, 1030–1034 (2016).

    Article  CAS  Google Scholar 

  10. Marguet, P., Tanouchi, Y., Spitz, E., Smith, C. & You, L. PLoS One 5, e11909 (2010).

    Article  Google Scholar 

  11. Garg, N., Manchanda, G. & Kumar, A. Antonie Van Leeuwenhoek 105, 289–305 (2014).

    Article  Google Scholar 

  12. Nordström, K. & Dasgupta, S. EMBO Rep. 7, 484–489 (2006).

    Article  Google Scholar 

  13. Rutledge, P.J. & Challis, G.L. Nat. Rev. Microbiol. 13, 509–523 (2015).

    Article  CAS  Google Scholar 

  14. Slager, J., Kjos, M., Attaiech, L. & Veening, J.W. Cell 157, 395–406 (2014).

    Article  CAS  Google Scholar 

  15. Stewart, P.S. & Franklin, M.J. Nat. Rev. Microbiol. 6, 199–210 (2008).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA

    Feilun Wu & Lingchong You

  2. Department of Molecular Genetics and Microbiology, Duke University School of Medicine and the Center for Genomic and Computational Biology, Duke University, Durham, North Carolina, USA

    Lingchong You

Authors
  1. Feilun Wu
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  2. Lingchong You
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Correspondence to Lingchong You.

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The authors declare no competing financial interests.

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Wu, F., You, L. Hacking DNA copy number for circuit engineering. Nat Genet 49, 1164–1165 (2017). https://doi.org/10.1038/ng.3923

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