Recent evidence suggests that a substantial portion of complex disease risk alleles modify gene expression in a cell-specific manner1,2,3,4. To identify candidate causal genes and biological pathways of immune-related complex diseases, we conducted expression quantitative trait loci (eQTL) analysis on five subsets of immune cells (CD4+ T cells, CD8+ T cells, B cells, natural killer (NK) cells and monocytes) and unfractionated peripheral blood from 105 healthy Japanese volunteers. We developed a three-step analytical pipeline comprising (i) prediction of individual gene expression using our eQTL database and public epigenomic data, (ii) gene-level association analysis and (iii) prediction of cell-specific pathway activity by integrating the direction of eQTL effects. By applying this pipeline to rheumatoid arthritis data sets, we identified candidate causal genes and a cytokine pathway (upregulation of tumor necrosis factor (TNF) in CD4+ T cells). Our approach is an efficient way to characterize the polygenic contributions and potential biological mechanisms of complex diseases.
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We would like to thank all the doctors and staff who participated in sample collection for eQTL analysis and the BioBank Japan Project and staff at the Laboratory for Genotyping Development. This research was supported by funding from Takeda pharmaceutical Co., Ltd. (Y. Kochi, K.F. and K. Yamamoto), and a grant from RIKEN (K. Ishigaki, Y. Kochi, A.S., Y.M., Y. Kamatani and M.K.). The BioBank Japan Project is supported by the Japanese Ministry of Education, Culture, Sports, Sciences and Technology.
The authors declare no competing financial interests.
Supplementary Figures 1–17 and Supplementary Tables 1 and 14 (PDF 5848 kb)
Enrichment of cell-specific eQTL variants within transcription factor binding sites. (XLSX 125 kb)
List of candidate causal genes identified by combining GWAS catalog and eQTL data of each cell type. (XLSX 21945 kb)
List of candidate causal genes identified by combining GWAS catalog and exon-level eQTL data of each cell type. (XLSX 3917 kb)
List of candidate causal genes identified by combining GWAS catalog and TSS-conditioned eQTL data of each cell type. (XLSX 1376 kb)
Bayesian test for colocalisation between GWAS variants of RA and eQTL variants of each cell type. (XLSX 14 kb)
eQTL variants and their effect sizes used to predict gene expression of CD4+ T cells. (XLSX 3086 kb)
eQTL variants and their effect sizes used to predict gene expression of CD8+ T cells. (XLSX 3034 kb)
eQTL variants and their effect sizes used to predict gene expression of B cells. (XLSX 4168 kb)
eQTL variants and their effect sizes used to predict gene expression of NK cells. (XLSX 3605 kb)
eQTL variants and their effect sizes used to predict gene expression of monocytes. (XLSX 5041 kb)
eQTL variants and their effect sizes used to predict gene expression of PB. (XLSX 4484 kb)
Genes with Bonferroni significance in the case-control analysis using predicted gene expression. (XLSX 13 kb)
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Ishigaki, K., Kochi, Y., Suzuki, A. et al. Polygenic burdens on cell-specific pathways underlie the risk of rheumatoid arthritis. Nat Genet 49, 1120–1125 (2017). https://doi.org/10.1038/ng.3885
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