Gene expression in mammals is precisely regulated by the combination of promoters and gene-distal regulatory regions, known as enhancers. Several studies have suggested that some promoters might have enhancer functions. However, the extent of this type of promoters and whether they actually function to regulate the expression of distal genes have remained elusive. Here, by exploiting a high-throughput enhancer reporter assay, we unravel a set of mammalian promoters displaying enhancer activity. These promoters have distinct genomic and epigenomic features and frequently interact with other gene promoters. Extensive CRISPR–Cas9 genomic manipulation demonstrated the involvement of these promoters in the cis regulation of expression of distal genes in their natural loci. Our results have important implications for the understanding of complex gene regulation in normal development and disease.
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We thank J.-C. Andrau and J. Imbert for critical reading of the manuscript. We thank the IBiSA 'Transcriptomics and Genomics Marseille-Luminy' (TGML) platform for sequencing of CapStarr-seq samples and the cell biology platform for management of cell culture. Work in the laboratory of S.S. was supported by recurrent funding from INSERM and Aix-Marseille University and by specific grants from the European Union's FP7 Programme (282510-BLUEPRINT), ARC (PJA 20151203149) and A*MIDEX (ANR-11-IDEX-0001-02). L.T.M.D., A.G. and G.C. were supported, respectively, by Vietnam International Education Development (911), CONACYT and FRM.
The authors declare no competing financial interests.
Integrated supplementary information
(a) Luciferase enhancer assays of proximal DHSs defined as active or inactive enhancers by STARR-seq in P5424 cells. For each candidate, both orientations were tested. Data represent the normalized fold change over the vector control. Error bars show s.d. from three independent transfections (***P < 0.001, **P < 0.01, *P < 0.1; two-sided Student’s t test). (b) Enrichment score of lymphoid transcription factors at distal and proximal DHSs based on ChIP–seq data from developing thymocytes. The enrichment score was calculated as the −log10 (P value) obtained with a hypergeometric test (depletion is represented by negative values).
Supplementary Figure 2 CapStarr-seq experimental control and epigenomic profiles of Epromoters in K562 and HeLa cells.
(a) IGV screenshots of STARR-seq signals for four STARR-seq-positive controls in HeLa cells. (b) FACS analysis of GFP expression in K562 (left) and HeLa (right) cells transfected with a human promoter library or empty vector. Controls were untransfected cells. The increase in GFP expression in transfected cells with the promoter library indicates potential enhancer activity in the pooled library. (c) Overlap with CpG islands (50%) and regions conserved in placental mammals (10%) using the EpiExplorer tool. The control is non-Epromoters with equal levels of gene expression as Epromoters in the same cell type. (d) Average profiles of epigenomic features for Epromoters and control promoters with the same expression pattern of associated genes. Statistical significance was calculated in a region centered on the TSS (±1 kb) using two-sided Mann–Whitney U tests. Only significant differences (P < 0.001) are shown. (e) Percentage of chromatin state 4 (strong enhancers) found in K562 Epromoters (left) and HeLa Epromoters (right) across ENCODE cell lines using the EpiExplorer tool. (f,g) Density plots of TSS positions corresponding to the selected promoter regions using CAGE peaks from ENCODE data in HeLa (f, top) and K562 (f, bottom) cells and data from FANTOM3 (g, top) and FANTOM5 (g, bottom) (Kolmogorov test). (h) Percentage of TSSs assigned to RefSeq-defined TSSs using different CAGE databases (from data in Supplementary Table 2b). (i) Comparison of the number of different RefSeq-defined TSSs per coding gene (one-sided Mann–Whitney U test).
(a) Cumulative plot of normalized RNA levels (FPKM) for genes from the IFN-αβ signaling pathway (Reactome), based on RNA–seq data from 23 cell lines. The HeLa and K562 cell lines are highlighted (Kolmogorov test). (b) Heat map showing RNA–seq relative expression (FPKM) for genes from the IFN-αβ signaling pathway (Reactome) expressed at significantly higher levels in HeLa cells as compared to the 22 remaining cell lines (SAM analysis; α = 0.5). (c) Transcription signatures related to stress/interferon response significantly enriched in the set of Epromoter-associated genes in HeLa cells (GREAT tool).
(a,b) Average profiles of ChIP–seq signals for ENCODE transcription factors enriched at Epromoters in K562 (a) and HeLa (b) cells. Statistical significances were calculated in a region centered on the TSS (±250 bp) using two-sided Mann–Whitney U tests.
(a,b) Heat maps showing the enrichment distribution (log2 (observed/expected)) of the non-redundant collection of motifs obtained by combining transcription factor binding motif (TFBM) databases (Jaspar vertebrates and Hocomoco Human). TFBMs were used to scan the extended Epromoter-associated TSS from −1 kb to +1 kb and clustering was performed based on the binding profiles in K562 (a) and HeLa (b) cells. Motifs enriched around the TSS (black line) were selected. (c,d) Significantly enriched motifs in K562 (c) and HeLa (d) cells were identified by comparing the binding enrichment within the promoter region (−200 bp to +50 bp with respect to the TSS; highlighted as orange boxes) between Epromoters and the non-Epromoters. Binding site distribution (left), motif logos (middle) and E values (right) are shown only for significantly enriched motifs (E < 0.001; χ2 test). (e) Enrichment of Epromoters and non-Epromoters as a function of the number of different TFBMs found. The enrichment score was calculated as the −log10 (P value) obtained by hypergeometric test.
(a) Scatterplots showing the Pearson correlation between the STARR-seq signal of Epromoters and the expression of associated genes. (b) Examples of consistent promoter–promoter interactions observed with different ChIA-PET data sets in K562 cells.
(a) General strategy for the generation of (E)promoter knockouts. Two gRNAs, G1 and G2, were designed flanking the genomic target to delete the intervening DNA segment. The CRISPR–Cas9 system creates two double-strand breaks (DSBs) at 3 - 4 nt upstream of the PAM sequences (red) and releases the excised DNA (purple). The resulting DSB is repaired by the NHEJ pathway. The genomic deletion is detected by PCR using primers P1 and P2. (b–h) Assessment of (E)promoter knockout. Left, IGV screenshots showing the DNase-seq (ENCODE) and RefSeq tracks for targeted regions. The locations of gRNAs (red boxes) and the expected sizes of deleted regions are indicated. Right, PCR validation of biallelic deletion in corresponding cell clones. Details on the gRNA sequences, PCR primers and expected PCR fragments are provided in Supplementary Table 9. (i) Strategy for the generation of the inverted FAF2 Epromoter knock-in. The two gRNAs, G1 and G2, used to generate DSBs are as in the knockout experiment. The repair template contains upstream and downstream homologous arms (HAs) flanking the inverted FAF2 Epromoter. The HDR-mediated repair pathway generates the inverted FAF2 Epromoter knock-in, which is detected by PCR with the combination of two primer pairs (1 + 2) and (2 + 3). (j) PCR validation of a successful inverted FAF2 Epromoter knock-in cell clone using the combination of primers shown in i. (k) RT–PCR detection of antisense (AS) transcription in an Inv.Ep.FAF2 clone. GAPDH was used as a cDNA loading control.
(a) Genomic tracks showing the 4C–seq analysis of interactions between the FAF2 (a) and RNF44 (b) promoters in WT and knockout K562 clones. The viewpoint from the FAF2 Epromoter is indicated by an arrowhead. The specific interaction between the FAF2 and RNF44 promoters is highlighted by the orange box. (b) UCSC Genome Browser tracks for H3K4me3, H3K27ac and H3K36me3 at the FAF2 locus and nearby regions across the HMEC, K562, HeLa and DND41 cell lines.
(a,b) IGV tracks for RNA–seq, ChIP–seq and ChIA-PET Pol II data in K562 and HeLa cells at the CSDE1 (a) and TAGLN2 (b) loci and nearby regions. The promoter–promoter interactions for Epromoters are highlighted in red. (c,d) qPCR analysis of gene expression in WT, ΔEp.CSDE1 (c) and ΔEp.TAGLN2 (d) clones. The number following the gene name is the number of independent cell clones. (e) Allelic frequency of the A versus T variant (chr1:160000435) in PIGM transcripts in WT, ΔEp.TAGLN2 homozygous and ΔEp.TAGLN2 heterozygous K562 clones. The total number of reads is indicated for each sample. The significant deviation of allelic frequency in heterozygous clones with respect to homozygous samples was calculated by performing a one-sided Student’s t test. (f) IGV screenshot showing tracks for RNA–seq, ChIP–seq and ChIA-PET Pol II data in K562 cells at the BAZ2B locus and the nearby region. (g) qPCR analysis of gene expression in WT and ΔEp.BAZ2B clones. Knockout of the BAZ2B Epromoter resulted in significant reduction of MARCH7 expression but had no effect on the nearby gene WDSUB1 or BAZ2B (using primers 1 and 2 shown in h). (h) UCSC Genome Browser tracks showing the different BAZ2B transcripts and primers used in g and i. (i) Alternative promoter usage for the BAZ2B gene was assessed by RT–PCR in K562 cells. The smaller fragment size observed in ΔEp.BAZ2B clones corresponds to the deletion of exon 1 (asterisk in h). (j) IGV tracks for FANTOM3 and ENCODE CAGE data, CapStarr-seq regions and RNA junctions around the TSS of the indicated gene. The red color in CapStarr-seq tracks represents active Epromoters. For c, d and g, error bars show s.d. (n = 3 independent RNA/cDNA preparation; ***P < 0.001, **P < 0.01, *P < 0.1, two-sided Student’s t test).
(a) Distribution of expression correlation for ChIA-PET interacting gene pairs including at least one Epromoter (red) or excluding Epromoters (gray) and randomly rewired gene pairs (green) using RNA–seq data from ENCODE. Statistical significance was assessed by Kolmogorov test. (b) Examples of clusters of interferon response genes (green labels) associated with Epromoters (red arrows) in HeLa cells. (c–e) qPCR analysis of gene expression in WT, ΔEp.YPEL4 (c,d) and ΔEp.METTL21A (e) cell clones. Error bars show s.d (n = 3 independent RNA/cDNA preparations). (f) Allelic frequency of the T versus C variant (chr11:57319339) in UBE2L6 transcripts in WT, ΔEp.YPEL4 homozygous and ΔEp.YPEL4 heterozygous K562 clones. The total number of reads is indicated for each sample. The significant deviation of allelic frequency in heterozygous clones with respect to homozygous samples was calculated by performing a one-sided Student’s t test.
Supplementary Figures 1–10 and Supplementary Note. (PDF 2563 kb)
Mouse DHS CapStarr-seq data. (XLSX 418 kb)
Human promoter CapStarr-seq data. (XLSX 7980 kb)
Functional enrichment of Epromoter-associated genes. (XLSX 18 kb)
Transcription factor enrichment. (XLSX 40 kb)
Summary of gene pairs with promoter–promoter interactions detected by ChIA-PET. (XLSX 361 kb)
List of gene-pairs with ChIA-PET interaction and involved in the interferon response. (XLSX 10 kb)
ChIA-PET gene pairs that harbor an eQTL within the ±1.5-kb expanded promoter region and that affect the expression of the distal gene. (XLSX 5009 kb)
Summary of CapStarr-seq sequencing data. (XLSX 8 kb)
Summary of ENCODE data used in this study. (XLSX 57 kb)
Oligonucleotide sequences used in this study. (XLSX 23 kb)
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Dao, L., Galindo-Albarrán, A., Castro-Mondragon, J. et al. Genome-wide characterization of mammalian promoters with distal enhancer functions. Nat Genet 49, 1073–1081 (2017) doi:10.1038/ng.3884
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