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Genome-wide association studies identify loci associated with age at menarche and age at natural menopause

Abstract

Age at menarche and age at natural menopause are associated with causes of substantial morbidity and mortality such as breast cancer and cardiovascular disease. We conducted a joint analysis of two genome-wide association studies of these two traits in a total of 17,438 women from the Nurses' Health Study (NHS, N = 2,287) and the Women's Genome Health Study (WGHS, N = 15,151). For age at menarche, we identified ten associated SNPs (P = 1 × 10−7–3 × 10−13) clustered at 6q21 (in or near the gene LIN28B) and 9q31.2 (in an intergenic region). For age at natural menopause, we identified 13 associated SNPs (P = 1 × 10−7–1 × 10−21) clustered at 20p12.3 (in the gene MCM8), 19q13.42 (in or near the gene BRSK1), 5q35.2 (in or near genes UIMC1 and HK3) and 6p24.2 (in the gene SYCP2L). These newly identified loci might expand understanding of the biological pathways regulating these two traits.

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Figure 1: Summary of GWA results by chromosome.
Figure 2: Detailed results for the six identified regions.

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Acknowledgements

We thank J. Miletich and A. Parker as well as the technical staff at Amgen for their collaboration and scientific support in performing the genotyping for the WGHS. The NHS GWAS was performed as part of the Cancer Genetic Markers of Susceptibility initiative of the NCI. We particularly acknowledge the contributions of R. Hoover, A. Hutchinson, K. Jacobs and G. Thomas. We thank J. Chen for discussion of gene functions. We thank H. Ranu and P. Soule for assistance. The WGHS is supported by HL 043851 and HL69757 from the National Heart, Lung, and Blood Institute and CA 047988 from the National Cancer Institute, the Donald W. Reynolds Foundation and the Fondation Leducq, with collaborative scientific support and funding for genotyping provided by Amgen. The NHS is supported by CA 40356 and U01-CA98233 from the National Cancer Institute. We acknowledge the study participants in the NHS and the WGHS for their contribution in making this study possible.

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Contributions

C.H., C.C., P.K. and D.I.C. performed the primary GWAS analyses in each study. C.H., C.C. and P.K. performed the joint analysis and contributed to the graphics supporting the figures. C.H., D.J.H. and D.I.C. wrote the manuscript with inputs from the other authors, especially S.J.C., P.K. and P.M.R. P.K., S.E.H. and D.J.H. are investigators of the NHS. J.E.B., G.P., P.M.R. and D.I.C. are investigators of the WGHS. All authors read and approved the final manuscript.

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Correspondence to Chunyan He.

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Supplementary Tables 1–4, Supplementary Figures 1 and 2 and Supplementary Methods (PDF 356 kb)

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He, C., Kraft, P., Chen, C. et al. Genome-wide association studies identify loci associated with age at menarche and age at natural menopause. Nat Genet 41, 724–728 (2009). https://doi.org/10.1038/ng.385

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