Supplementary Figure 15: Reprogramming of the TOP2B locus in response to 6AN. | Nature Genetics

Supplementary Figure 15: Reprogramming of the TOP2B locus in response to 6AN.

From: Epigenomic reprogramming during pancreatic cancer progression links anabolic glucose metabolism to distant metastasis

Supplementary Figure 15

(a) 6AN did not alter mRNA expression (top) or levels of H3K9me2 and H3K27ac (bottom) across the TOP2B genomic locus in the peritoneal (A38Per) subclone (ChIP–qPCR primer positions are shown with respect to the TOP2B gene). (b) In contrast, 6AN selectively repressed mRNA expression (n = 4 technical replicates; *P < 0.002, two-tailed t test) and induced enrichment of H3K9me2 across the TOP2B locus in the lung metastatic subclone matched from the same patient (A38Lg). (c,d) Representative RT–qPCR (A38Lg) verified RNAi knockdown of CDH2 and TOP2B with minimal effect on VIM (vimentin) expression. n = 3 technical replicates, each normalized to ERK; error bars, s.d.

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