Supplementary Figure 3: Specificity of chromatin modifications within heterochromatin domains. | Nature Genetics

Supplementary Figure 3: Specificity of chromatin modifications within heterochromatin domains.

From: Epigenomic reprogramming during pancreatic cancer progression links anabolic glucose metabolism to distant metastasis

Supplementary Figure 3

(a) Plots of ChIP–seq read densities normalized to input for histone modifications (y axes) showed that heterochromatin modifications (H3K9me2, H3K9me3, H3K27me3) were enriched in regions that were called heterochromatin (0% to 100%; bottom labels) for each subclone (indicated above). In contrast, euchromatin modifications (H3K36me3, H3K27ac) were depleted from the same regions. (b) Heterochromatin domains detected using alternative thresholds for domain calls produced similar results as shown in Figure 2a, demonstrating that the domain calls and reprogramming changes within them were robust across multiple sensitivity criteria (removal of short peaks less than 10 kb in length and merging of broad peaks separated by 15 kb are shown; other sensitivity tests using alternative thresholds produced nearly identical results that even more closely matched those in Fig. 2a).

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