Abstract
We analyzed the DNA methylome of ten subpopulations spanning the entire B cell differentiation program by whole-genome bisulfite sequencing and high-density microarrays. We observed that non-CpG methylation disappeared upon B cell commitment, whereas CpG methylation changed extensively during B cell maturation, showing an accumulative pattern and affecting around 30% of all measured CpG sites. Early differentiation stages mainly displayed enhancer demethylation, which was associated with upregulation of key B cell transcription factors and affected multiple genes involved in B cell biology. Late differentiation stages, in contrast, showed extensive demethylation of heterochromatin and methylation gain at Polycomb-repressed areas, and genes with apparent functional impact in B cells were not affected. This signature, which has previously been linked to aging and cancer, was particularly widespread in mature cells with an extended lifespan. Comparing B cell neoplasms with their normal counterparts, we determined that they frequently acquire methylation changes in regions already undergoing dynamic methylation during normal B cell differentiation.
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Acknowledgements
We thank C. López-Otín for critical reading of this manuscript, M. Dabad Castellà for his assistance with the WGBS data analysis and M.A. Peinado (Institute of Predictive and Personalized Medicine of Cancer, Barcelona) for providing RNA from HCT116 DKO cells. This work was funded by the European Union's Seventh Framework Programme through the Blueprint Consortium (grant agreement 282510) and the Spanish Ministry of Economy and Competitivity (MINECO; project SAF2009-08663). Methylation microarrays were outsourced to the Spanish Centro Nacional de Genotipado (CEGEN-ISCIII). We are indebted to the Genomics core facility of the Institut d'Investigacions Biomédiques August Pi i Sunyer (IDIBAPS) for technical help. This work was partially developed at the Centro Esther Koplowitz (CEK; Barcelona, Spain). M.K. is supported by Agéncia de Gestió d'Ajuts Universitaris i de Recerca (AGAUR; Generalitat de Catalunya), E.C. is an Academia Researcher of the Institució Catalana de Recerca i Estudis Avançats and J.I.M.-S. is a Ramón y Cajal researcher of MINECO.
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M.K., A.C.Q., N.R., M.P., X.A., F.P., D.A., B.P., G. Caron, T.F., M.O.M., M.E.F., S.-T.L. and J.L.W. provided samples from healthy donors and/or purified B cell subpopulations. M.K., A.C.Q., G. Castellano, R.B. and G. Clot analyzed DNA methylation and gene expression arrays. L.A., J.B. and M.G. performed WGBS library preparation and sequencing. A.M., S.H., R.P.S., E.R., A.E. and M.D.-F. processed and analyzed WGBS data. M.K., N.V.-D. and R.V.-B. performed validation experiments. M.K., G. Castellano. S.E., V.P., D. Rico and A.V. functionally characterized dynamically methylated genes. D. Richardson, L.C., A.D. and P.F. were in charge of data management. I.G.G. and H.G.S. coordinated sequencing efforts and performed primary data analysis. H.G.S., R.S., R.K. and E.C. participated in the study design and data interpretation. J.I.M.-S. conceived the study. J.I.M.-S. led the experiments and wrote the manuscript with predominant assistance from M.K. and R.B.
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Supplementary Text and Figures
Supplementary Figures 1–26 and Supplementary Tables 1–3. (PDF 2463 kb)
Supplementary Data Set 1
Annotation of CpGs belonging to each of the methylation modules detected by microarrays. (XLSX 7664 kb)
Supplementary Data Set 2
Enrichment analysis of transcription factor binding sites in the 20 DNA methylation modules. (XLSX 144 kb)
Supplementary Data Set 3
Enrichment analysis of transcription factor binding sites using differentially methylated CpGs identified by WGBS. (XLSX 61 kb)
Supplementary Data Set 4
Gene Ontology analysis of dynamic CpGs belonging to 20 main modules. (XLSX 46 kb)
Supplementary Data Set 5
Differential methylation analysis of B cell neoplasms and their normal cell counterparts. (XLSX 7982 kb)
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Kulis, M., Merkel, A., Heath, S. et al. Whole-genome fingerprint of the DNA methylome during human B cell differentiation. Nat Genet 47, 746–756 (2015). https://doi.org/10.1038/ng.3291
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DOI: https://doi.org/10.1038/ng.3291
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